Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin

doi:10.1080/10498850.2015.1011797

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin
	Yan, MY ; Qin, S ; Li, BF
刊名	JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
	2017
卷号	26 期号:10 页码:1166-1174
关键词	Purification structural aspect collagen walleye pollock fish skin
ISSN号	1049-8850
DOI	10.1080/10498850.2015.1011797
产权排序	[Yan, Mingyan ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China ; [Li, Bafang] Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
文献子类	Article
英文摘要	Type I collagen was extracted from walleye pollock (Theragra chalcogramma) skin and purified by DEAE-52 cellulose chromatography and gel filtration chromatography with Sephacryl S-300 HR. Fourier transform infrared spectroscopy spectra and X-ray diffraction pattern showed the existence of a helical arrangement, with the distance between the molecular chains of 1.18 nm and the unit height, typical of the triple helix, of 0.27 nm. Scanning electron microscopy (SEM) revealed the collagen had a filamentary structure. Peptide mapping, obtained by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), indicated that peptides with the molecular weight of 1,470, 1,565, 1,570, 2,150, and 2,470 Da were the major products of trypsin digestion of pollock skin collagen.
WOS关键词	Purification ; structural aspect ; collagen ; walleye pollock ; fish skin
WOS研究方向	Food Science & Technology
语种	英语
WOS记录号	WOS:000419358200007
资助机构	National Natural Science Foundation of China [31201455, 41576155]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24366]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China; 2.Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yan, MY,Qin, S,Li, BF. Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin[J]. JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY,2017,26(10):1166-1174.
APA	Yan, MY,Qin, S,&Li, BF.(2017).Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin.JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY,26(10),1166-1174.
MLA	Yan, MY,et al."Purification and Structural Aspects of Type I Collagen from Walleye Pollock (Theragra chalcogramma) Skin".JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY 26.10(2017):1166-1174.