渤海沉积物产脂肪酶细菌的筛选及其多样性分析

doi:DOI:10.13344/j.microbiol.china.170034

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	渤海沉积物产脂肪酶细菌的筛选及其多样性分析
	伍朝亚 ; 孟宪刚 ; 李岩 ; 曲慧敏 ; 解志红
刊名	微生物学通报
	2017-05-25
卷号	44 期号:7 页码:1655-1667
关键词	Marine sediment Lipase-producing bacteria Diversity Enzyme activity Bohai Sea 海洋沉积物产脂肪酶细菌多样性酶活渤海
ISSN号	0253-2654
DOI	DOI:10.13344/j.microbiol.china.170034
其他题名	Screening and diversity of lipase-producing bacteria in the sediments of Bohai Sea
产权排序	(1) 兰州交通大学化学与生物工程学院 ; (2) 中国科学院烟台海岸带研究所
文献子类	Article
英文摘要	[Objective] To uncover the diversity of cultivable lipase-producing bacterial isolated from the sediments of Bohai Sea and expand our knowledge on lipase-producing bacteria. Through screening efficient lipase production strains to expanding the marine lipase-producing microorganism resources.【Methods】 The lipase-producing bacteria were isolated by using tween-80 plate and tributyrin plate from 8 sediment samples of the Bohai Sea. The diversity of these bacteria was evaluated through phylogenetic analyses based on 16S rRNA gene sequences. Using the p-nitrophenol method to detect extracellular lipase activity and select high lipase producing strains. [Results] A total of 51 lipase-producing strains were isolated from 8 sediment samples. The isolates were classified into 8 genera of three phyla including Bacteroidetes, Proteobacteria, and Firmicutes, with Pseudoalteromonas (35.2%), Marinobacter (23.5%) and Sulfitobacter (17.6%) as the dominant. Enzyme activity test showed that all the strains were able to secrete lipase, and a lipase-producing strain Marinobacter sp.70623 possesses the highest lipase activity of 42.4 U/mL. [Conclusion] The cultivable lipase-producing bacteria isolated from the sediment of Bohai Sea are abundant with Pseudoalteromonas, Marinobacter and Sulfitobacter bacteria as the dominant groups, all the tested strains possess lipase activity, and the highest lipase activity producing strain Marinobacter sp. 70623 was obtained in this study.
WOS关键词	Microbiology
WOS研究方向	Microbiology ; 微生物学
语种	中文
CSCD记录号	CSCD:6023843
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24250]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.兰州交通大学化学与生物工程学院; 2.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	伍朝亚,孟宪刚,李岩,等. 渤海沉积物产脂肪酶细菌的筛选及其多样性分析[J]. 微生物学通报,2017,44(7):1655-1667.
APA	伍朝亚,孟宪刚,李岩,曲慧敏,&解志红.(2017).渤海沉积物产脂肪酶细菌的筛选及其多样性分析.微生物学通报,44(7),1655-1667.
MLA	伍朝亚,et al."渤海沉积物产脂肪酶细菌的筛选及其多样性分析".微生物学通报 44.7(2017):1655-1667.