A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I

doi:10.1039/c8an02022a

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I
	Fu, XL ; Wang, YQ ; Liu, YM ; Liu, HT ; Fu, LW ; Wen, JH ; Li, JW ; Wei, PH ; Chen, LX
刊名	ANALYST
	2019-03-07
卷号	144 期号:5 页码:1582-1589
关键词	ACUTE MYOCARDIAL-INFARCTION DISPERSED CARBON NANOTUBES PEROXIDASE-LIKE CATALYSIS SURFACE-ENHANCED RAMAN ULTRASENSITIVE DETECTION COLORIMETRIC SENSOR OPTICAL NANOPROBES SILVER DEPOSITION ASSAY BIOSENSORS
ISSN号	0003-2654
DOI	10.1039/c8an02022a
产权排序	[Fu, Xiuli ; Liu, Yongming ; Liu, Huitao ; Wen, Jiahui ; Li, Jingwen ; Chen, Lingxin] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China ; [Wang, Yunqing ; Fu, Longwen ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Wei, Peihai] Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
文献子类	Article
英文摘要	Cardiac troponin I (cTnI) was considered as the gold standard for acute myocardial infarction (AMI) diagnosis owing to its superior cardiac specificity for cardiac damage and showing little or no changes in patients with a skeletal muscle disease or trauma. Herein, a new signal amplification surface-enhanced Raman scattering (SERS) platform was developed for recognition and detection of cTnI by using gold nanoparticles (AuNPs), graphene oxide (GO) and magnetic beads (MB). Here, antibody/Raman reporter labeled AuNP-functionalized GO were employed as both SERS nanotags and signal amplification carriers. Monoclonal antibody modified MB were applied as the capture probe and separation agents. In the presence of cTnI, sandwich type immunocomplexes, capture probe/target/SERS nanotags, were formed through antibody-antigen-antibody interactions. Due to the strong SERS enhancement ability of the designed GO/AuNP complexes and a high binding chance between cTnI and the GO/AuNP complexes, the proposed SERS-based immunoassay could selectively detect cTnI with a high sensitivity (detection limit of 5 pg mL(-1)) and a good linearity was obtained in a range of 0.01-1000 ng mL(-1). In addition, this method was also successfully applied for detecting cTnI in serum substitute media with a similar linear range. Furthermore, this strategy can be constructed with different kinds of antibodies and Raman reporters, and thus can be easily used for simultaneous detection of multiple biomarkers. Therefore, this proposed SERS-based signal amplification technique shows strong potential for the clinical diagnosis of AMI disease.
WOS关键词	ACUTE MYOCARDIAL-INFARCTION ; DISPERSED CARBON NANOTUBES ; PEROXIDASE-LIKE CATALYSIS ; SURFACE-ENHANCED RAMAN ; ULTRASENSITIVE DETECTION ; COLORIMETRIC SENSOR ; OPTICAL NANOPROBES ; SILVER DEPOSITION ; ASSAY ; BIOSENSORS
WOS研究方向	Chemistry, Analytical
语种	英语
WOS记录号	WOS:000461614000008
资助机构	National Natural Science Foundation of Shandong Province in China [ZR2017BB026] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21275158, 21575159] ; Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [ZR2014BL031]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24976]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 3.Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Fu, XL,Wang, YQ,Liu, YM,et al. A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I[J]. ANALYST,2019,144(5):1582-1589.
APA	Fu, XL.,Wang, YQ.,Liu, YM.,Liu, HT.,Fu, LW.,...&Chen, LX.(2019).A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I.ANALYST,144(5),1582-1589.
MLA	Fu, XL,et al."A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I".ANALYST 144.5(2019):1582-1589.