Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice

doi:10.1016/j.snb.2018.11.056

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice
	Zhang, Xiaoyu ; He, Na ; Huang, Yan ; Yu, Fabiao ; Li, Bowei ; Lv, Changjun ; Chen, Lingxin
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2019-03-01
卷号	282 页码:69-77
关键词	Fluorescent probes Near-infrared fluorescence Cysteine Mitochondria target Lung cancer
ISSN号	0925-4005
DOI	10.1016/j.snb.2018.11.056
产权排序	[Zhang, Xiaoyu ; He, Na ; Lv, Changjun] Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China ; [Zhang, Xiaoyu ; He, Na ; Huang, Yan ; Li, Bowei ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China ; [Zhang, Xiaoyu ; He, Na ; Yu, Fabiao ; Lv, Changjun] Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China ; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
文献子类	Article
英文摘要	Cysteine (Cys) plays significant roles in many physiological processes, although its normal concentration is maintained at the micromole level. Abnormally high levels of intracellular Cys can lead to many diseases including cancer. Recent years, many effective fluorescent probes have been developed for the selective detection of Cys against other biological thiols. Herein, we synthesized a ratiometric near-infrared (NIR) fluorescent probe Cy-OAcr for selective imaging of intracellular Cys. Cy-OAcr has a lipophilic iminium cation unit as the mitochondrial guider and an acrylate group as the Cys recognition unit as well as a fluorescence modulator for rearranging the conjugated Tc-electron system of cyanine fluorophore. Upon detection of Cys, there occurs a significant absorption and fluorescence spectral shift, which are desirably beneficial for ratiometric detection. This probe has high sensitivity and selectivity for Cys detection over glutathione (GSH), homocysteine (Hcy), and other biomolecules with a low limit of detection at 0.09 mu M. Probe Cy-OAcr is capable to detect and image Cys in three living cancer cell lines and their corresponding tumor-bearing mice models. More importantly, we successfully apply this fluorescent probe to evaluate the level of Cys in orthotopic lung cancer model. Imaging analyses reveal that the probe can discriminate tumor lesions from normal tissues, indicating its significant potential applications for clinical auxiliary diagnosis of cancer.
WOS关键词	TURN-ON PROBE ; HYDROGEN-SULFIDE ; IN-VIVO ; ENDOGENOUS CYSTEINE ; OXIDATIVE STRESS ; LIVING CELLS ; GLUTATHIONE ; HOMOCYSTEINE ; EMISSION ; THIOLS
WOS研究方向	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000455090000009
资助机构	National Nature Science Foundation of China [21775162, 21864011, 31470415, 81670064, 21575159, 41776110] ; Talent Program of Hainan Medical University [XRC180006, XRC180007] ; Hundred-Talent Program (Hainan) ; Medical Science and Technology Development Project of Shandong Province [2017WS800] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24640]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China; 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 3.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China; 4.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Xiaoyu,He, Na,Huang, Yan,et al. Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,282:69-77.
APA	Zhang, Xiaoyu.,He, Na.,Huang, Yan.,Yu, Fabiao.,Li, Bowei.,...&Chen, Lingxin.(2019).Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice.SENSORS AND ACTUATORS B-CHEMICAL,282,69-77.
MLA	Zhang, Xiaoyu,et al."Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice".SENSORS AND ACTUATORS B-CHEMICAL 282(2019):69-77.