Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe

doi:10.1039/c8tb01794h

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe
	Gao, Min ; Wang, Rui ; Yu, Fabiao ; Li, Bowei ; Chen, Lingxin
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2018-11-07
卷号	6 期号:41 页码:6637-6645
关键词	HYDROGEN POLYSULFIDES LIVING CELLS ZEBRAFISH EMBRYOS CYSTEINE SULFIDE H2S PERSULFIDE CONSTRUCTION MODULATION CHEMISTRY
ISSN号	2050-750X
DOI	10.1039/c8tb01794h
产权排序	[Gao, Min ; Wang, Rui ; Yu, Fabiao ; Li, Bowei ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Wang, Rui ; Yu, Fabiao] Hainan Med Univ, Inst Funct Mat & Mol Imaging, Coll Emergency & Trauma, Haikou 571199, Hainan, Peoples R China ; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
文献子类	Article
英文摘要	Hypoxia is a significant global issue affecting the health of organisms. Oxygen homeostasis is critical for mammalian cell survival and cellular activities. Hypoxic stress can lead to cell injury and death, which contributes to many diseases. Sulfane sulfur is involved in crucial roles in physiological processes of maintaining intracellular redox state and ameliorating oxidative damage. Therefore, real-time imaging of changes in sulfane sulfur levels is important for understanding their biofunctions in cells. In this study, we develop a new near-infrared (NIR) fluorescent probe BD-diSeH for imaging of sulfane sulfur changes in cells and in vivo under hypoxic stress. The probe includes two moieties: an NIR azo-BODIPY fluorophore equipped with a strong nucleophilic phenylselenol group (-SeH). The probe is capable of tracing dynamic changes of endogenous sulfane sulfur based on a fast and spontaneous intramolecular cyclization reaction. The probe has been successfully used for imaging sulfane sulfur in 3D-multicellular spheroid and mouse hippocampus under hypoxic stress. The overall levels of sulfane sulfur are affected by the degree and length of hypoxic stress. The results reveal a close relationship between sulfane sulfur and hypoxia in living cells and in vivo, allowing better understanding of physiological and pathological processes involving sulfane sulfur. Moreover, to investigate the effects of environmental hypoxia on aquatic animals, this probe has been applied for sulfane sulfur detection in hypoxic zebrafish.
WOS关键词	HYDROGEN POLYSULFIDES ; LIVING CELLS ; ZEBRAFISH EMBRYOS ; CYSTEINE ; SULFIDE ; H2S ; PERSULFIDE ; CONSTRUCTION ; MODULATION ; CHEMISTRY
WOS研究方向	Materials Science, Biomaterials
语种	英语
WOS记录号	WOS:000448340500015
资助机构	National Nature Science Foundation of China [21775162, 41776110, 21575159] ; program of Youth Innovation Promotion Association, CAS [2015170] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24573]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 3.Hainan Med Univ, Inst Funct Mat & Mol Imaging, Coll Emergency & Trauma, Haikou 571199, Hainan, Peoples R China; 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Min,Wang, Rui,Yu, Fabiao,et al. Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(41):6637-6645.
APA	Gao, Min,Wang, Rui,Yu, Fabiao,Li, Bowei,&Chen, Lingxin.(2018).Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe.JOURNAL OF MATERIALS CHEMISTRY B,6(41),6637-6645.
MLA	Gao, Min,et al."Imaging of intracellular sulfane sulfur expression changes under hypoxic stress via a selenium-containing near-infrared fluorescent probe".JOURNAL OF MATERIALS CHEMISTRY B 6.41(2018):6637-6645.