Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking

doi:10.3389/fmicb.2018.02115

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking
	Lv, Min ; Lo, Ching ; Hsu, Cheng-Chih ; Wang, Yuwen ; Chiang, Yin-Ru ; Sun, Qian ; Wu, Yang ; Li, Yan ; Chen, Lingxin ; Yu, Chang-Ping
刊名	FRONTIERS IN MICROBIOLOGY
	2018-09-07
卷号	9 页码:2115
关键词	metoprolol (MPL) microalgae enantioselective biodegradation transformation products biodegradation pathway MS/MS spectral similarity networking
ISSN号	1664-302X
DOI	10.3389/fmicb.2018.02115
产权排序	[Lv, Min ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China ; [Lv, Min ; Yu, Chang-Ping] Natl Taiwan Univ, Grad Inst Environm Engn, Taipei, Taiwan ; [Lo, Ching ; Hsu, Cheng-Chih] Natl Taiwan Univ, Dept Chem, Taipei, Taiwan ; [Wang, Yuwen ; Sun, Qian ; Wu, Yang ; Li, Yan] Chinese Acad Sci, Inst Urban Environm, CAS Key Lab Urban Pollutant Convers, Xiamen, Peoples R China ; [Chiang, Yin-Ru] Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan
文献子类	Article
英文摘要	Metoprolol (MPL) is a chiral beta-blocker ubiquitously detected in various environments due to its low to moderate removal in wastewater treatment plants. This study was conducted to test the potential of using microalgae to degrade emerging contaminant MPL and to characterize the enantiomeric enrichment during MPL degradation by microalgae. The results showed that PO43--P, NO3--N and MPL could be simultaneously removed in the synthetic effluent by the targeted microalgae species, indicating microalgae were promising in wastewater treatment. Stereoselectivity was observed during MPL degradation by microalgae, with R-form enrichment. A marginal linear relationship between MPL degradation and enantiomeric enrichment was observed, implying that the enantiomeric tool, used as a quantitative indicator of biodegradation, could possibly be applied in MPL degradation by microalgae. An efficient liquid chromatograph tandem high resolution mass spectrometry (LC-HRMS/MS) chiral analytical method was developed to identify transformation products (TPs). The results showed that MS/MS spectral similarity networking could be used as a powerful tool to quickly identify unknown TPs. A total of 6 pairs of chiral TPs were identified, among which two new TPs of MPL including hydroxy{4-[2-hydroxy-3-(isopropylamino)propoxy]phenyl}acetic acid (alpha-HMPLA) and 4-[2-Hydroxy-3-(isopropylamino)propoxy]benzaldehyde (DMPLD) were firstly reported, and proposed transformation pathways of MPL by microalgae were given. Considering the paired TPs detected and that the degradation of the two enantiomers followed first order kinetics, the two enantiomers likely had the same degradation mechanism.
WOS关键词	PERSONAL CARE PRODUCTS ; WATER TREATMENT PLANTS ; WASTE-WATER ; MASS-SPECTROMETRY ; OXIDATIVE-METABOLISM ; ILLICIT DRUGS ; PHARMACEUTICALS ; REMOVAL ; CHINA ; BIODEGRADATION
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000443981000001
资助机构	MOST, Taiwan [105-2113-M-002-004-MY2, 105-2221-E-002-009-MY3] ; Center for Emerging Materials and Advanced Devices, National Taiwan University [NTU-ERP-106R880211, 106R880219] ; NSFC [41601525] ; NSF of Shandong province [ZR2016DB07]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24531]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Acad Sinica, Biodivers Res Ctr, Taipei, Taiwan 2.Chinese Acad Sci, Inst Urban Environm, CAS Key Lab Urban Pollutant Convers, Xiamen, Peoples R China; 3.Natl Taiwan Univ, Dept Chem, Taipei, Taiwan; 4.Natl Taiwan Univ, Grad Inst Environm Engn, Taipei, Taiwan; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China;
推荐引用方式 GB/T 7714	Lv, Min,Lo, Ching,Hsu, Cheng-Chih,et al. Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking[J]. FRONTIERS IN MICROBIOLOGY,2018,9:2115.
APA	Lv, Min.,Lo, Ching.,Hsu, Cheng-Chih.,Wang, Yuwen.,Chiang, Yin-Ru.,...&Yu, Chang-Ping.(2018).Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking.FRONTIERS IN MICROBIOLOGY,9,2115.
MLA	Lv, Min,et al."Identification of Enantiomeric Byproducts During Microalgae-Mediated Transformation of Metoprolol by MS/MS Spectrum Based Networking".FRONTIERS IN MICROBIOLOGY 9(2018):2115.