Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea

doi:10.1016/j.scitotenv.2020.137712

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea
	Zhang, Yuxuan ; Lu, Jian ; Wu, Jun ; Wang, Jianhua ; Lin, Yichen
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-06-10
卷号	720 页码:137712
关键词	RIVER CHINA ABUNDANCE POLLUTION IMPACTS ESTUARY WATER FATE ARGS
DOI	10.1016/j.scitotenv.2020.137712
产权排序	[Zhang, Yuxuan ; Lu, Jian ; Wang, Jianhua ; Lin, Yichen] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China ; [Zhang, Yuxuan ; Lu, Jian ; Lin, Yichen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Lu, Jian] Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China ; [Wu, Jun] Ludong Univ, Sch Resources & Environm Engn, Yantai 264025, Shandong, Peoples R China
文献子类	Article
英文摘要	Extensive and improper overuse of antibiotics resulted in the prevalence of antibiotic resistance genes (ARGs). As the typical semi-enclosed continental shelf sea, the Bohai Sea has been considered as one of the most polluted marine areas in China. However, no comprehensive investigation on the spatial distribution of ARGs in sediments from the Bohai Sea has been reported. A large-scale sampling was performed in the Bohai Sea areas. The abundances of ARGs (6 classes, 29 ARG subtypes), class 1 integron-integrase gene (intI1), hmt-DNA and 16S rRNA gene were evaluated. IntI1 was detected with higher abundances in coastal areas ranging from 2.8 x 10(5) to 2.5 x 10(8) copies/g. The total ARGs abundances varied over 3 orders of magnitude in different sampling sites with the maximum at 4.9 x 10(8) copies/g. Sulfonamides resistance genes were ubiquitous and abundant with the abundances ranging from 5.7 x 10(4) to 1.8 x 10(7) copies/g, and quinolones resistance genes varied greatly in different samples. The contour map demonstrated that ARGs were more abundant in the Laizhou Bay, the south of Bohai Bay and the eastern of central sea basin. Most of the target ARG subtypes were detected with 100% detection frequencies. The genes of sul1, sul2 and tetX were detected with both higher absolute and relative abundance, while the abundance of beta-lactams ARG subtypes was lower. Principal component analysis (PCA) and redundancy analysis (RDA) indicated that no significant differences in the ARGs abundance existed in different samples, and the sediment qualities played important roles in the distribution of ARGs. Bacterial communities were investigated and 768 strong and significant connections between ARGs and bacteria were identified. The possible hosts of ARGs were revealed by network analysis with higher relative abundance in coastal areas than the sea. (C) 2020 Elsevier B.V. All rights reserved.
WOS关键词	RIVER ; CHINA ; ABUNDANCE ; POLLUTION ; IMPACTS ; ESTUARY ; WATER ; FATE ; ARGS
语种	英语
WOS记录号	WOS:000525736600104
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41877131] ; Taishan Scholars Project of Shandong Province [tsqn201812116] ; One Hundred Talents Program of Chinese Academy of SciencesChinese Academy of Sciences [Y629041021] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-QYZX-114] ; Two Hundred Talents Plan of Yantai [Y739011021] ; Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25020]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Ludong Univ, Sch Resources & Environm Engn, Yantai 264025, Shandong, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Yuxuan,Lu, Jian,Wu, Jun,et al. Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,720:137712.
APA	Zhang, Yuxuan,Lu, Jian,Wu, Jun,Wang, Jianhua,&Lin, Yichen.(2020).Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea.SCIENCE OF THE TOTAL ENVIRONMENT,720,137712.
MLA	Zhang, Yuxuan,et al."Occurrence and distribution of antibiotic resistance genes in sediments in a semi-enclosed continental shelf sea".SCIENCE OF THE TOTAL ENVIRONMENT 720(2020):137712.