Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis

doi:10.1016/j.jip.2019.107215

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis
	Lv, JS ; Zhao, JM ; Yang, DL ; Wu, HF ; Cong, M
刊名	JOURNAL OF INVERTEBRATE PATHOLOGY
	2019-09
卷号	166 页码:107215
关键词	Mytilus galloprovincialis Mytimacin-4 Tissue distribution Recombinant proteins Antimicrobial activity
ISSN号	0022-2011
DOI	10.1016/j.jip.2019.107215
产权排序	[Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China ; [Zhao, Jianmin ; Yang, Dinglong ; Wu, Huifeng ; Cong, Ming] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
文献子类	Article
英文摘要	Antimicrobial peptides (AMPs) play fundamental roles in the innate immunity of invertebrates. Mytimacin-4 is a kind of AMP gene previously sequenced from Mytilus galloprovincialis based on an identified EST sequence in our lab. In the present study, the tissue distribution and antimicrobial activities of mytimacin-4 were further investigated. A qRT-PCR analysis revealed that mytimacin-4 transcripts were constitutively expressed in all of the tested tissues of M. galloprovincialis, with the highest expression level in the posterior adductor muscle. After challenge by Vibrio anguillarum, the expression level of mytimacin-4 gene was significantly increased at 24 h (P < 0.05) in the mantle and increased at 48 h (P < 0.05) in the posterior adductor muscle. This finding suggested that mytimacin-4 transcripts were inducible upon pathogen infection. A minimal inhibitory concentration (MIC) assay indicated that recombinant mytimacin-4 protein had potent antimicrobial activities against gram-positive and gram-negative bacteria. Among the tested microorganisms, mytimacin-4 protein exhibited strong inhibition activities against Bacillus subtilis and Vibrio parahaemolyticus with MICs of 0.315 mu M and 0.62 mu M respectively. This study provides for the first time direct evidence of antimicrobial action of mytimacin-4 in M. galloprovincialis.
WOS关键词	RICH ANTIMICROBIAL PEPTIDE ; MOLECULAR CHARACTERIZATION ; ANTIBACTERIAL PEPTIDES ; HEMOCYTES ; INVERTEBRATES ; CHALLENGE ; SEAWATER ; IMMUNITY ; BINDING ; LEECH
WOS研究方向	Zoology
语种	英语
WOS记录号	WOS:000485335900010
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 41876135]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24867]
专题	海岸带生物资源高效利用研究与发展中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China; 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Lv, JS,Zhao, JM,Yang, DL,et al. Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2019,166:107215.
APA	Lv, JS,Zhao, JM,Yang, DL,Wu, HF,&Cong, M.(2019).Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis.JOURNAL OF INVERTEBRATE PATHOLOGY,166,107215.
MLA	Lv, JS,et al."Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis".JOURNAL OF INVERTEBRATE PATHOLOGY 166(2019):107215.