Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen

doi:10.1016/j.aquatox.2017.11.012

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen
	Cong, Ming ; Wu, Huifeng ; Cao, Tengfei ; Lv, Jiasen ; Wang, Qing ; Ji, Chenglong ; Li, Chenghua ; Zhao, Jianmin
刊名	AQUATIC TOXICOLOGY
	2018-01
卷号	194 页码:121-131
关键词	Ruditapes philippinarum Digital gene expression Transcriptome Ammonia nitrogen Toxicity
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2017.11.012
产权排序	[Cong, Ming ; Wu, Huifeng ; Wang, Qing ; Ji, Chenglong ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China ; [Cao, Tengfei ; Li, Chenghua] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China ; [Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
文献子类	Article
英文摘要	Previous study revealed severe toxic effects of ammonia nitrogen on Ruditapes philippinanun including lysosomal instability, disturbed metabolic profiles, gill tissues with damaged structure, and variation of neurotransmitter concentrations. However, the underlying molecular mechanism was not fully understood yet. In the present study, digital gene expression technology (DGE) was applied to globally screen the key genes and pathways involved in the responses to short- and long-term exposures of ammonia nitrogen. Results of DGE analysis indicated that short-term duration of ammonia exposure affected pathways in Dorso-ventral axis formation, Notch signaling, thyroid hormone signaling and protein processing in endoplasmic reticulum. The long-term exposure led to DEGs significantly enriched in gap junction, immunity, signal and hormone transduction, as well as key substance metabolism pathways. Functional research of significantly changed DEGs suggested that the immunity of R. philippinarum was weakened heavily by toxic effects of ammonia nitrogen, as well as neuro-transduction and metabolism of important substances. Taken together, the present study provides a molecular support for the previous results of the detrimental toxicity of ammonia exposure in R. philippinarum, further work will be performed to investigate the specific genes and their certain functions involved in ammonia toxicity to molluscs.
WOS关键词	BINDING-PROPERTIES ; CHLAMYS-FARRERI ; TOXICITY ; CALCIUM ; FISH ; STABILITY ; RECEPTOR ; GENOME ; STRESS ; LECTIN
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
WOS记录号	WOS:000423644200014
资助机构	Natural Science Foundation of China [41406132] ; National Key Basic Research Program of China [2015CB453303] ; Youth Innovation Promotion Association CAS
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24406]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室海岸带生物资源高效利用研究与发展中心
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; 2.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;
推荐引用方式 GB/T 7714	Cong, Ming,Wu, Huifeng,Cao, Tengfei,et al. Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen[J]. AQUATIC TOXICOLOGY,2018,194:121-131.
APA	Cong, Ming.,Wu, Huifeng.,Cao, Tengfei.,Lv, Jiasen.,Wang, Qing.,...&Zhao, Jianmin.(2018).Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen.AQUATIC TOXICOLOGY,194,121-131.
MLA	Cong, Ming,et al."Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen".AQUATIC TOXICOLOGY 194(2018):121-131.