Potentiometric aptasensing of small molecules based on surface charge change

doi:10.1016/j.snb.2017.12.067

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Potentiometric aptasensing of small molecules based on surface charge change
	Lv, Enguang ; Ding, Jiawang ; Qin, Wei
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-04-15
卷号	259 页码:463-466
关键词	Aptasensor Bisphenol A Potentiometry Surface charge Solid state
ISSN号	0925-4005
DOI	10.1016/j.snb.2017.12.067
产权排序	[Lv, Enguang ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China ; [Lv, Enguang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Solid-state potentiometric sensors based on surface charge change are well-established tools for measuring ions and biological species. However, their use for detection of small molecules with high sensitivity and good selectivity is still elusive. In this work, a novel potentiometric aptasensing platform for small molecules is presented, using bisphenol A (BPA) as a model. The proposed sensor can be prepared by layer-by-layer assembling of carboxylated multiwall carbon nanotubes, poly(diallyldimethylammonium chloride) (polycation), and aptamer (polyanion) on the electrode surface. The presence of BPA induces the conformational change and detachment of the aptamer at the surface of the modified electrode, which leads to a variation of the surface charge (negative to positive) and therefore a potential change. The introduction of polyions can cause substantial charge change on the electrode surface, thus improving the sensitivity of the sensor. The morphology and electron-transfer properties of the electrode have been characterized. Under optimum conditions, the present sensor shows a stable response to BPA in the concentration range from 3.2 x 10(-8) to 1.0 x 10(-6) M with a detection limit of 1.0 x 10(-8) M. The proposed methodology can be used for sensitive potentiometric sensing of other small molecules involved in aptamer/target binding events. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词	ION-SELECTIVE ELECTRODES ; MEMBRANE ELECTRODES ; BISPHENOL-A ; SENSORS ; GOLD ; COPPER(II) ; ADSORPTION ; FILMS
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000424877600055
资助机构	National Natural Science Foundation of China [21575158, 21475148] ; National Key Research and Development Program of China [2016YFC1400700] ; Instrument Developing Project of the Chinese Academy of Sciences [Y728021021] ; CAS Youth Innovation Promotion Association [2014190] ; Taishan Scholar Program of Shandong Province [TS20081159]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24375]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Lv, Enguang,Ding, Jiawang,Qin, Wei. Potentiometric aptasensing of small molecules based on surface charge change[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,259:463-466.
APA	Lv, Enguang,Ding, Jiawang,&Qin, Wei.(2018).Potentiometric aptasensing of small molecules based on surface charge change.SENSORS AND ACTUATORS B-CHEMICAL,259,463-466.
MLA	Lv, Enguang,et al."Potentiometric aptasensing of small molecules based on surface charge change".SENSORS AND ACTUATORS B-CHEMICAL 259(2018):463-466.