毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素

doi:10.3724/SP.J.1123.2018.01011

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素
	高芳芳 ; 刘军英 ; 鹿文慧 ; 李金花 ; 刘惠涛
刊名	色谱
	2018-06-08
卷号	36 期号:06 页码:573-577
关键词	毛细管电泳压力辅助电动进样胶束电动色谱酚类雌激素水样
ISSN号	1000-8713
DOI	10.3724/SP.J.1123.2018.01011
其他题名	Determination of four phenolic estrogens in water samples by pressure-assisted electrokinetic injection coupled with capillary electrophoresis
产权排序	(1)烟台大学化学化工学院 ; (2)中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室
英文摘要	A capillary electrophoresis (CE) method was developed for the simultaneous separation and determination of four phenolic estrogens (PEs), in which pressure-assisted electrokinetic injection (PAEKI) was adopted as the on-line concentration method to enrich the PEs. Several parameters affecting PAEKI conditions such as injection voltage and injection time, were systematically investigated and compared with the usual parameters. Under the optimized PAEKI conditions (-9 kV, 0.3 psi (ca. 2.1 kPa), 0.4 min), the four PEs separated completely within 7 min. Good linearities were attained in the range of 0.05-5 mg/L for hexestrol and dienestrol, and 0.1-10 mg/L for bisphenol A and diethylstilbestrol, with correlation coefficients (r) over 0.9936. The limits of detection (S/N=3) were 0.0071-0.017 mg/L, and enrichment factors ranged from 11 to 15 compared to normal hydrodynamic injection. The combined micellar electrokinetic chromatographic-PAEKI method developed was used to determine the PEs in tap water and lake water samples; limits of quantification (S/N=10) of 0.029-0.064 mg/L and 0.033-0.079 mg/L were attained, respectively, by the two sample types. Furthermore, recoveries ranged from 75.6% to 110.1% with relative standard deviations (n=5) within 4.6%-11.8%. To use this PAEKI method, researchers would only need to adjust the parameters of the CE apparatus to perform on-line enrichment of analytes, without using other reagents; this demonstrates the simplicity, rapidity, and highly automated nature of this method.
资助项目	National Natural Science Foundation of China[21477160]
语种	中文
CSCD记录号	CSCD:6275211
资助机构	国家自然科学基金项目#21477160
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24329]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.烟台大学化学化工学院; 2.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室
推荐引用方式 GB/T 7714	高芳芳,刘军英,鹿文慧,等. 毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素[J]. 色谱,2018,36(06):573-577.
APA	高芳芳,刘军英,鹿文慧,李金花,&刘惠涛.(2018).毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素.色谱,36(06),573-577.
MLA	高芳芳,et al."毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素".色谱 36.06(2018):573-577.