Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

doi:10.1016/j.jhazmat.2020.125023

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level
	Wang, Qiaoning 2,5; Wang, Hongdan 2; Jiang, Yaru 2; Lv, Min 2; Wang, Xiaoyan 1; Chen, Lingxin 2,3,4
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2021-06-05
卷号	411 页码:10
关键词	Sulfamethoxazole Vibrio diabolicus strain L2-2 Transcriptome Biotransformation Resistance
ISSN号	0304-3894
DOI	10.1016/j.jhazmat.2020.125023
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Sulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1-10 mu g/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 mu g/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2-2 could be activated under SMX stress of 10 mu g/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics.
资助项目	National Natural Science Foundation of China[2196209] ; National Natural Science Foundation of China[21876199] ; National Natural Science Foundation of China[21804010] ; National Natural Science Foundation of China[41601525] ; Taishan Scholar Project Special Funding of China[ts20190962]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000638080300006
资助机构	National Natural Science Foundation of China ; Taishan Scholar Project Special Funding of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27226]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, Lingxin
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res,Res Ctr Coastal Envi, Yantai 264003, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,et al. Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,411:10.
APA	Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,Lv, Min,Wang, Xiaoyan,&Chen, Lingxin.(2021).Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level.JOURNAL OF HAZARDOUS MATERIALS,411,10.
MLA	Wang, Qiaoning,et al."Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level".JOURNAL OF HAZARDOUS MATERIALS 411(2021):10.