Potential risks of microplastics combined with superbugs: Enrichment of antibiotic resistant bacteria on the surface of microplastics in mariculture system

doi:10.1016/j.ecoenv.2019.109852

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Potential risks of microplastics combined with superbugs: Enrichment of antibiotic resistant bacteria on the surface of microplastics in mariculture system
	Yuxuan Zhang; Lu J(吕剑); Jun Wu
刊名	Ecotoxicology and Environmental Safety
	2020
卷号	187 期号:0 页码:109852
关键词	Microplastic Antibiotic resistant bacteria Superbugs Multi-drug resistance Antibiotic resistance genes Mariculture
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2019.109852
产权排序	[Zhang, Yuxuan ; Lu, Jian ; Wang, Jianhua ; Luo, Yongming] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China ; [Zhang, Yuxuan ; Lu, Jian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Lu, Jian] Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China ; [Wu, Jun] Ludong Univ, Sch Resources & Environm Engn, Yantai 264025, Shandong, Peoples R China
文献子类	Article
英文摘要	Microplastics have become emerging pollutants and served as potential vectors for harmful bacteria, while rare information on the emergency and propagation of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) on the surface of microplastics is available. This study investigated the enrichment of ARB, especially multi-antibiotic resistant bacteria (MARS), on the surface of microplastics in mariculture system. Polyethylene terephthalate accounted for the highest proportion (75%) in the collected microplastics. The counts of cultivable ARB in microplastic samples were 6.40 x 10(6)-2.48 x 10(8) cfu/g, which were 100-5000 times higher than those in water samples. The ratios of cultivable ARB to total cultivable bacteria from microplastic samples were higher than those from water samples. High-throughput sequencing showed that the diversity and abundance of cultivable ARB in the microplastic samples was high with the predominant bacterial genera of Vibrio, Muricauda and Ruegeria. Total 160 MARB isolates were obtained and most of isolates were obtained from the microplastic samples. MARB isolates resisting or intermediating to four and three antibiotics accounted for much higher proportions in the microplastic samples, and the higher percentage of antibiotic resistance was to penicillin, sulfafurazole, erythromycin and tetracycline. The dominant multiple antibiotic resistance profile was TET-SFX-ERY-PEN, which accounted for 25.4% in microplastic samples and 23.9% in water samples. In typical MARB isolates, the positive detection rate of ARGs was up to 80.0% in microplastic samples while that was 65.3% in water samples. Five types of class 1 integrons (intI1) associated gene cassette arrays and seven types of gene cassettes were detected in microplastic samples, which were more than those in water samples. These results revealed that microplastics were hazardous pollutants for the enrichment of ARB, especially superbugs, and the spread of antibiotic resistance.
WOS关键词	SP NOV. ; GENES ; POLLUTANTS ; PLASTICS ; HEALTH
WOS研究方向	Environmental Sciences ; Toxicology
语种	英语
WOS记录号	WOS:000500410800038
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41877131] ; Taishan Scholar Program of Shandong Province [tsqn201812116] ; One Hundred Talents Program of Chinese Academy of SciencesChinese Academy of Sciences [Y629041021] ; Two -Hundred Talents Plan of Yantai [Y739011021]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24187]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Lu J(吕剑)
推荐引用方式 GB/T 7714	Yuxuan Zhang,Lu J,Jun Wu. Potential risks of microplastics combined with superbugs: Enrichment of antibiotic resistant bacteria on the surface of microplastics in mariculture system[J]. Ecotoxicology and Environmental Safety,2020,187(0):109852.
APA	Yuxuan Zhang,Lu J,&Jun Wu.(2020).Potential risks of microplastics combined with superbugs: Enrichment of antibiotic resistant bacteria on the surface of microplastics in mariculture system.Ecotoxicology and Environmental Safety,187(0),109852.
MLA	Yuxuan Zhang,et al."Potential risks of microplastics combined with superbugs: Enrichment of antibiotic resistant bacteria on the surface of microplastics in mariculture system".Ecotoxicology and Environmental Safety 187.0(2020):109852.