Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging

doi:10.1016/j.molstruc.2019.01.026

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	Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging
	Liu, JH ; Chen, XY ; Yang, XQ ; Chen, J ; Qi, ZM
刊名	JOURNAL OF MOLECULAR STRUCTURE
	2019-04-15
卷号	1182 页码:119-122
关键词	Astaxanthin Carotenogenesis FTIR microspectroscopy imaging Haematococcus pluvialis Inhibitors
ISSN号	0022-2860
DOI	10.1016/j.molstruc.2019.01.026
产权排序	[Liu, Jinghua ; Chen, Xinyi] Anhui Sci & Technol Univ, Coll Life & Hlth Sci, Fengyang 233100, Peoples R China ; [Yang, Xianqing] Minist Agr Peoples Republ China, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China ; [Chen, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Qi, Zeming] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
文献子类	Article
英文摘要	Haematococcus pluvialis has emerged as a promising microalga for its rapid accumulation of astaxanthin. However, the underlying mechanism for astaxanthin biosynthesis is still unclear and needs further exploring in terms of the chemical changes of algal cell during the carotenogenesis pathway. In this study, the chemical changes were monitored in the algal cells exposed to carotenogenesis inhibitors by Fourier-transform infrared (FTIR) microspectroscopy imaging. The significant increase of signals at 3010 and 2925 cm(-1) suggests the accumulation of carotenoids during the algal cell encysting. In contrast, a relatively low level of carotenoids in the algal cells contacted to nicotine and diphenylamine were observed for the biosynthesis blocking of beta-carotene and astaxanthin. The decrease of the absorbance at 1740 and 1650 cm(-1) indicates that lipids, proteins and astaxanthin biosynthesis were also inhibited by nicotine and diphenylamine while the cell wall biosynthesis was not disturbed by the inhibitors for the strong absorbance band at 1035 cm(-1) assigned to polysaccharides. This work demonstrates that FTIR microspectroscopy imaging is an effective and none-invasive tool to analyze chemical changes and the components location in the single algal cell with high spatial resolution and it facilitates the study of carotenoid biosynthetic pathway. (C) 2019 Elsevier B.V. All rights reserved.
WOS关键词	GREEN-ALGA ; CULTIVATION ; LIGHT ; IR
WOS研究方向	Chemistry, Physical
语种	英语
WOS记录号	WOS:000458618500015
资助机构	Fund of Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, China [NYJG201802] ; Research Projects of Anhui Science and Technology University for Key Discipline [AKZDXK2015B02]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24958]
专题	烟台海岸带研究所_中科院烟台海岸带研究所知识产出
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 2.Anhui Sci & Technol Univ, Coll Life & Hlth Sci, Fengyang 233100, Peoples R China; 3.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China 4.Minist Agr Peoples Republ China, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China;
推荐引用方式 GB/T 7714	Liu, JH,Chen, XY,Yang, XQ,et al. Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging[J]. JOURNAL OF MOLECULAR STRUCTURE,2019,1182:119-122.
APA	Liu, JH,Chen, XY,Yang, XQ,Chen, J,&Qi, ZM.(2019).Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging.JOURNAL OF MOLECULAR STRUCTURE,1182,119-122.
MLA	Liu, JH,et al."Astaxanthin accumulation in Haematococcus pluvialis observed through Fourier-transform infrared microspectroscopy imaging".JOURNAL OF MOLECULAR STRUCTURE 1182(2019):119-122.