Super-resolution and optical sectioning integrated structured illumination microscopy
Dan, Dan1; Gao, Peng2; Zhao, Tianyu1,3; Dang, Shipei1,3; Qian, Jia1,3; Lei, Ming1; Min, Junwei1; Yu, Xianghua1; Yao, Baoli1,3
刊名JOURNAL OF PHYSICS D-APPLIED PHYSICS
2021-02-18
卷号54期号:7
关键词structured illumination microscopy super-resolution optical sectioning
ISSN号0022-3727;1361-6463
DOI10.1088/1361-6463/abc4a8
产权排序1
英文摘要

Super-resolution structured illumination microscopy (SR-SIM) has attracted a great deal of attention in the past few decades. As a wide-field imaging technique, SR-SIM usually suffers from issues relating to out-of-focus background, particularly when imaging thick samples. In this study, we develop an integrated SIM with simultaneous SR and optical sectioning (OS) capabilities, facilitating SR imaging of stacked optical sections, with the out-of-focus background suppressed. The combination of the merits of SR and OS is realized by means of a new image reconstruction algorithm. We confirm the validity of the integrated SIM, both experimentally and in simulation. We anticipate that this integrated SIM will assist biologists in obtaining much clearer SR images in relation to thick specimens.

语种英语
出版者IOP PUBLISHING LTD
WOS记录号WOS:000595248500001
内容类型期刊论文
源URL[http://ir.opt.ac.cn/handle/181661/94032]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Yao, Baoli
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
2.Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Dan, Dan,Gao, Peng,Zhao, Tianyu,et al. Super-resolution and optical sectioning integrated structured illumination microscopy[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS,2021,54(7).
APA Dan, Dan.,Gao, Peng.,Zhao, Tianyu.,Dang, Shipei.,Qian, Jia.,...&Yao, Baoli.(2021).Super-resolution and optical sectioning integrated structured illumination microscopy.JOURNAL OF PHYSICS D-APPLIED PHYSICS,54(7).
MLA Dan, Dan,et al."Super-resolution and optical sectioning integrated structured illumination microscopy".JOURNAL OF PHYSICS D-APPLIED PHYSICS 54.7(2021).
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