Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway

doi:10.1088/1361-6463/aba56f

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 健康与医学技术研究所

	Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway
	Ma, Jie 4,5; Yu, K. N.6,7; Zhang, Hao 2; Nie, Lili 4,5; Cheng, Cheng 1; Cui, Shujun 4; Yang, Miaomiao 3,4; Chen, Guodong 4,5; Han, Wei 4,5
刊名	JOURNAL OF PHYSICS D-APPLIED PHYSICS
	2020-11-11
卷号	53
关键词	non-thermal plasma apoptosis protective autophagy c-JUN N-terminal kinase Sestrin2
ISSN号	0022-3727
DOI	10.1088/1361-6463/aba56f
通讯作者	Ma, Jie()
英文摘要	Although non-thermal plasma (NTP) has been proved to be an effective way to kill cancer cells, the mechanism of NTP-induced cancer cell death is still not clear. In this study, we found that NTP exposure caused reactive oxygen species generation and apoptosis in A549 and MDA-MB-231 cells. Meanwhile, NTP treatment also induced autophagy, as evidenced by the formation of acidic vesicular organelles and conversion of LC3-I to LC3-II. Suppression of autophagy by chloroquine significantly increased NTP-induced cell death, indicating that NTP-induced autophagy acted in a protective role from apoptosis. Furthermore, NTP treatment significantly increased Sestrin2 (Sesn2) expression and activated the JNK signaling pathway. Knocking down Sesn2 with special siRNA enhanced NTP-induced cell death, while pretreatment with JNK inhibitor abolished the increase of Sesn2 and LC3 formation, and promoted cell apoptosis induced by NTP. These indicated that the JNK/Sesn2 pathway was involved in autophagy and apoptosis induced by NTP. These findings provided evidence that supplement with autophagy inhibition might be a useful strategy to improve the tumor cell killing effect of NTP.
资助项目	National Key R&D Program of China[2019YFC0119000] ; Natural Science Fund of Anhui Province[2008085QH352] ; Chinese National Natural Science Foundation[81573093] ; Chinese National Natural Science Foundation[U1632145] ; CASHIPS Director's Fund[YZJJ2018QN19] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong[IRF/0024]
WOS关键词	CELL-DEATH ; ATMOSPHERIC PLASMA ; OXIDATIVE STRESS ; CANCER ; INDUCTION ; SESTRIN2 ; SURVIVAL ; AKT
WOS研究方向	Physics
语种	英语
出版者	IOP PUBLISHING LTD
WOS记录号	WOS:000563484600001
资助机构	National Key R&D Program of China ; Natural Science Fund of Anhui Province ; Chinese National Natural Science Foundation ; CASHIPS Director's Fund ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/92793]
专题	健康与医学技术研究所
通讯作者	Ma, Jie
作者单位	1.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 2.Xi An Jiao Tong Univ, Ctr Plasma Biomed, State Key Lab Elect Insulat & Power Equipment, Xian 710049, Shanxi, Peoples R China 3.Anhui Med Univ, Clin Pathol Ctr, Affiliated Hosp 4, Hefei 230022, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China 5.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China 6.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China 7.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China
推荐引用方式 GB/T 7714	Ma, Jie,Yu, K. N.,Zhang, Hao,et al. Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS,2020,53.
APA	Ma, Jie.,Yu, K. N..,Zhang, Hao.,Nie, Lili.,Cheng, Cheng.,...&Han, Wei.(2020).Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway.JOURNAL OF PHYSICS D-APPLIED PHYSICS,53.
MLA	Ma, Jie,et al."Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway".JOURNAL OF PHYSICS D-APPLIED PHYSICS 53(2020).