Ratiometric Fluorescent Biosensor for Visual Discrimination of Cancer Cells with Different Telomerase Expression Levels | |
He, Changtian1,3; Liu, Zhengjie1,3; Wu, Qilong1,3; Zhao, Jun2,3; Liu, Renyong2,3; Liu, Bianhua2,3; Zhao, Tingting2,3 | |
刊名 | ACS SENSORS |
2018-04-01 | |
卷号 | 3期号:4页码:757-762 |
关键词 | ratiometric fluorescent probe telomerase activity detection cancer cell discrimination naked eye FRET |
ISSN号 | 2379-3694 |
DOI | 10.1021/acssensors.8b00059 |
英文摘要 | Telomerase is inactive in normal somatic cells but highly activated in tumor cells to maintain their indefinite proliferation and immortal phenotype. As a specific marker for the generation and progress of almost all tumors, the detection of telomerase activity by classical PCR techniques has served in the biological research of tumors. However, the detection of in situ telomerase activity in cell extracts to evaluate the malignancy, progress, and metastasis of tumors remains a daunting challenge. Here, a precisely designed FRET-based ratiometric fluorescent oligonucleotide probe has achieved high-fidelity detection of telomerase activity for accurate discrimination of different cancer cells toward advanced diagnosis of tumors. Our method is superior to other methods in its capabilities to quantify telomerase activity in cell extracts and visualize various tumor cell extracts with different telomerase expression levels by the naked eye for clinical diagnosis. In particular, the ratiometric fluorescent probe used in the assay could exclude other experimental factors influence, and further avoid false positive signal generation. The method reported here could provide a reliable, accurate, and convenient way in medical diagnostics and therapeutic response assessment. |
资助项目 | National Natural Science Foundation of China[21475135] ; National Natural Science Foundation of China[21335006] ; National Natural Science Foundation of China[61705239] ; National Natural Science Foundation of China[21375131] ; National Natural Science Foundation of China[21703255] ; Natural Science Foundation of Anhui Province[1708085MB35] ; Natural Science Foundation of Anhui Province[1608085QB32] |
WOS关键词 | ELECTROCHEMICAL BIOSENSOR ; SENSITIVE DETECTION ; IMMORTAL CELLS ; MESSENGER-RNA ; QUANTUM DOTS ; DNA ; ASSAY ; FRET |
WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000431165200004 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/36245] |
专题 | 合肥物质科学研究院_中科院合肥智能机械研究所 |
通讯作者 | Zhao, Tingting |
作者单位 | 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | He, Changtian,Liu, Zhengjie,Wu, Qilong,et al. Ratiometric Fluorescent Biosensor for Visual Discrimination of Cancer Cells with Different Telomerase Expression Levels[J]. ACS SENSORS,2018,3(4):757-762. |
APA | He, Changtian.,Liu, Zhengjie.,Wu, Qilong.,Zhao, Jun.,Liu, Renyong.,...&Zhao, Tingting.(2018).Ratiometric Fluorescent Biosensor for Visual Discrimination of Cancer Cells with Different Telomerase Expression Levels.ACS SENSORS,3(4),757-762. |
MLA | He, Changtian,et al."Ratiometric Fluorescent Biosensor for Visual Discrimination of Cancer Cells with Different Telomerase Expression Levels".ACS SENSORS 3.4(2018):757-762. |
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