Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells

doi:10.21037/atm-20-4667

	Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells
	Qin, Feng 3,4,5,6; Fan, Qiang 3,4; Yu, Peter K. N.2,8; Almahi, Waleed Abdelbagi 3,4; Kong, Peizhong 3,4; Yang, Miaomiao 3,4,9; Cao, Wei 3,4; Nie, Lili 3,5; Chen, Guodong 3,5; Han, Wei 1,3,5,7
刊名	ANNALS OF TRANSLATIONAL MEDICINE
	2021-04-01
卷号	9
关键词	Breast cancer acquired radioresistance gene expression profiling energy metabolism
ISSN号	2305-5839
DOI	10.21037/atm-20-4667
通讯作者	Chen, Guodong(c3024034@163.com) ; Han, Wei(hanw@hfcas.ac.cn)
英文摘要	Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels. Methods: We established an acquired radioresistant cell line from its parental NF639 cell line (HER2-positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. RNA-sequencing (RNA-seq) was also used to reveal the potential regulating genes and molecular mechanisms associated with the acquired changed phenotypes. Real-time PCR was used to validate the results of RNA-seq. Results: The NF639R cells exhibited increased radioresistance and enhanced activity of proliferation, migration and anti-apoptosis, but decreased basal ROS. Two main energy metabolism pathways, mitochondrial respiration and glycolytic, were also upregulated. Furthermore, 490 differentially expressed genes were identified by RNA-seq. Enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes showed many differently expressed genes were significantly enriched in cell morphology, proliferation, migration, anti-apoptosis, antioxidation, tumor stem cells and energy metabolism and the signaling cascades such as the transforming growth factor-beta, Wnt, Hedgehog, vascular endothelial growth factor, retinoic acid-inducible gene I (RIG-I)-like receptor, Toll-like receptor and nucleotide oligomerization domain (NOD)-like receptor were significantly altered in NF639R cells. Conclusions: In clinical radiotherapy, repeat radiotherapy for short-term recurrence of breast cancer may result in enhanced radioresistance and promote malignant progression. Our research provided hints to understand the tumor resistance to radiotherapy de novo and recurrence with a worse prognosis following radiotherapy. Keywords:
资助项目	Chinese National Natural Science Foundation[81703168] ; Chinese National Natural Science Foundation[U1632145] ; Chinese National Natural Science Foundation[81227902] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2019HSCCIP010] ; Natural Science Fund of Anhui Province[1608085QH181] ; PriorityAcademic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong[IRF/0024]
WOS关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; PATTERN-RECOGNITION RECEPTORS ; TUMOR-CELLS ; CELLULAR-RESPONSES ; ENERGY-METABOLISM ; DOSE RADIATION ; GROWTH ; CARCINOMA ; EMT ; PROLIFERATION
WOS研究方向	Oncology ; Research & Experimental Medicine
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000647719000025
资助机构	Chinese National Natural Science Foundation ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Natural Science Fund of Anhui Province ; PriorityAcademic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122152]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Guodong; Han, Wei
作者单位	1.Soochow Univ, Collaborat Innovat Ctr Radiat Med, Jiangsu Higher Educ Inst, Suzhou, Peoples R China 2.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Peoples R China 4.Grad Sch USTC, Sci Isl Branch, Hefei, Peoples R China 5.Chinese Acad Sci, Hefei Canc Hosp, Hefei, Peoples R China 6.Anhui Acad Agr Sci, Inst Sericultural, Hefei, Peoples R China 7.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Peoples R China 8.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China 9.Anhui Med Univ, Affiliated Hosp 4, Clin Pathol Ctr, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Qin, Feng,Fan, Qiang,Yu, Peter K. N.,et al. Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021,9.
APA	Qin, Feng.,Fan, Qiang.,Yu, Peter K. N..,Almahi, Waleed Abdelbagi.,Kong, Peizhong.,...&Han, Wei.(2021).Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells.ANNALS OF TRANSLATIONAL MEDICINE,9.
MLA	Qin, Feng,et al."Properties and gene expression profiling of acquired radioresistance in mouse breast cancer cells".ANNALS OF TRANSLATIONAL MEDICINE 9(2021).