Gradient radiation breeding and culture domestication of menaquinone producing strains

doi:10.1007/s00449-021-02508-8

	Gradient radiation breeding and culture domestication of menaquinone producing strains
	Wu, Hefang 1,2; Wang, Han 2; Wang, Peng 2; Zhao, Geihai 2; Liu, Hui 2; Wang, Li 2; Sun, Xiaowen 2; Zheng, Zhiming 2
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2021-05-18
关键词	Biofilm lipid peroxidation Gamma ray mutagenesis Low-energy ion implantation Menaquinone (MK) SOD activity
ISSN号	1615-7591
DOI	10.1007/s00449-021-02508-8
通讯作者	Zheng, Zhiming(zhengzhiming2014@163.com)
英文摘要	By comparing the survival rate and positive mutation rate of the primary mutagenic strain and progeny mutagenic strain under different radiation doses, the results showed that the tolerance of the mutagenic strain to radiation dose increased with the increase of the mutagenic generations. We adopted an improved gradient radiation breeding strategy to improve the breeding efficiency. The strains were treated with radiation in four stages. The first stage was low energy N+ ion implantation (ion energy 15 keV, dose 80 x 2.6 x 10(13) cm(-2)). In the second stage, the energy and dose of N+ ion reached to 20 keV, 90 x 2.6 x 10(13) cm(-2). In the third stage, Co-60-gamma radiation (dose of 1.56 kGy) was used. In the fourth stage, the radiation dose of Co-60-gamma increased to 1.82 kGy. After each stage of radiation, the MK (Menaquinone) precursor 1, 4-dihydroxy-2-naphthalate (DHNA) was used as the stress factor to domesticate the mutant strains. By gradually increasing the concentration of DHNA in the culture medium, the substrate tolerance of Flavobacterium sp. was effectively improved. By measuring SOD (superoxide dismutase) activity and malondialdehyde, it showed that the cell damage caused by radiation mutagenesis to the offspring mutant was less than that of the primary mutant. Changes in membrane permeability and membrane potential of the mutant strains were reflected in changes in fluorescence intensity of luciferin diacetate and rhodamine 123, which could explain the enhanced substrate tolerance of strain F-2. After gradient radiation breeding and culture acclimation, the biomass of mutant Strain F-2 was 6.59 g/L, and the MK yield was 9.59 mg/L.
资助项目	Hi-Tech Research and Development Program of China (key 863 Fund of China)[2014AA021704] ; Natural Science Foundation of Anhui Province[1308085MA07] ; Natural Science Foundation of Anhui Province[1608085QC46]
WOS关键词	SUPEROXIDE-DISMUTASE ; HUMAN OSTEOBLASTS ; VITAMIN-K ; APOPTOSIS ; MUTATION ; FOOD
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000651666500001
资助机构	Hi-Tech Research and Development Program of China (key 863 Fund of China) ; Natural Science Foundation of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122119]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Zhiming
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Shushanhu Rd 350, Hefei 210031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wu, Hefang,Wang, Han,Wang, Peng,et al. Gradient radiation breeding and culture domestication of menaquinone producing strains[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2021.
APA	Wu, Hefang.,Wang, Han.,Wang, Peng.,Zhao, Geihai.,Liu, Hui.,...&Zheng, Zhiming.(2021).Gradient radiation breeding and culture domestication of menaquinone producing strains.BIOPROCESS AND BIOSYSTEMS ENGINEERING.
MLA	Wu, Hefang,et al."Gradient radiation breeding and culture domestication of menaquinone producing strains".BIOPROCESS AND BIOSYSTEMS ENGINEERING (2021).