Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

doi:10.1080/15548627.2020.1797280

	Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
	Klionsky, Daniel J.2324,2325; Abdel-Aziz, Amal Kamal 2326,2327; Abdelfatah, Sara 2328; Abdellatif, Mahmoud 2329; Abdoli, Asghar 2330; Abel, Steffen 2331; Abeliovich, Hagai 2332; Abildgaard, Marie H.1448,2333; Abudu, Yakubu Princely 2334; Acevedo-Arozena, Abraham 2335,2336,2337
刊名	AUTOPHAGY
	2021
卷号	17
关键词	Autophagosome cancer flux LC3 lysosome macroautophagy neurodegeneration phagophore stress vacuole
ISSN号	1554-8627
DOI	10.1080/15548627.2020.1797280
通讯作者	Klionsky, Daniel J.(klionsky@umich.edu)
英文摘要	In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
资助项目	Biotechnology and Biological Sciences Research Council[BBS/E/B/000C0413] ; British Heart Foundation[PG/17/14/32867] ; Medical Research Council[MR/M00869X/2] ; Medical Research Council[G0501003] ; Medical Research Council[MR/R025096/1] ; Medical Research Council[MR/R009732/1] ; Medical Research Council[MR/S032304/1] ; Medical Research Council[MR/S009426/1] ; Medical Research Council[MR/N022696/1] ; NCI NIH HHS[R01 CA181196] ; NCI NIH HHS[R01 CA197398] ; NCI NIH HHS[R01 CA190370] ; NCI NIH HHS[R01 CA200310] ; NCI NIH HHS[K08 CA230151] ; NCI NIH HHS[R01 CA233794] ; NCI NIH HHS[R01 CA244144] ; NCI NIH HHS[R01 CA247992] ; NEI NIH HHS[R01 EY029675] ; NEI NIH HHS[R01 EY019643] ; NEI NIH HHS[R01 EY026885] ; NEI NIH HHS[P30 EY010572] ; NEI NIH HHS[R01 EY027733] ; NHLBI NIH HHS[R01 HL107594] ; NHLBI NIH HHS[R01 HL088256] ; NHLBI NIH HHS[R01 HL131667] ; NHLBI NIH HHS[R01 HL118558] ; NHLBI NIH HHS[R01 HL123340] ; NHLBI NIH HHS[R01 HL072166] ; NHLBI NIH HHS[R01 HL154147] ; NHLBI NIH HHS[R01 HL153614] ; NHLBI NIH HHS[R01 HL085629] ; NHLBI NIH HHS[R01 HL141759] ; NIAID NIH HHS[R01 AI136921] ; NIAID NIH HHS[R01 AI121244] ; NIAID NIH HHS[R01 AI139046] ; NIAID NIH HHS[K08 AI102971] ; NIAMS NIH HHS[R01 AR077440] ; NIAMS NIH HHS[R01 AR061370] ; NIA NIH HHS[P01 AG054407] ; NIA NIH HHS[R01 AG049780] ; NIA NIH HHS[R01 AG062375] ; NIA NIH HHS[R01 AG062475] ; NIDDK NIH HHS[R01 DK107220] ; NIDDK NIH HHS[R01 DK112698] ; NIDDK NIH HHS[R01 DK099558] ; NIDDK NIH HHS[R01 DK121545] ; NIDDK NIH HHS[R01 DK093668] ; NIDDK NIH HHS[R01 DK108921] ; NIGMS NIH HHS[R35 GM122536] ; NIGMS NIH HHS[R35 GM127029] ; NIGMS NIH HHS[R35 GM130331] ; NIGMS NIH HHS[R35 GM119571] ; NIGMS NIH HHS[R35 GM131919] ; NIGMS NIH HHS[R15 GM102846] ; NIGMS NIH HHS[R01 GM118660] ; NIGMS NIH HHS[R01 GM115517] ; NINDS NIH HHS[R01 NS091218] ; NINDS NIH HHS[R25 NS070682] ; NINDS NIH HHS[P01 NS097197] ; NINDS NIH HHS[R01 NS103981] ; NINDS NIH HHS[R01 NS115876] ; BLRD VA[I01 BX004235] ; BLRD VA[I01 BX003803] ; BLRD VA[I01 BX004444] ; Pancreatic Cancer UK[FLF2015_02_BARTS]
WOS关键词	CHAPERONE-MEDIATED AUTOPHAGY ; ACTIVATED PROTEIN-KINASE ; PROGRAMMED CELL-DEATH ; STARVATION-INDUCED AUTOPHAGY ; ENDOPLASMIC-RETICULUM STRESS ; NF-KAPPA-B ; BREAST-CANCER CELLS ; LIFE-SPAN EXTENSION ; AMYOTROPHIC-LATERAL-SCLEROSIS ; GENOME-WIDE ASSOCIATION
WOS研究方向	Cell Biology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000636121800001
资助机构	Biotechnology and Biological Sciences Research Council ; British Heart Foundation ; Medical Research Council ; NCI NIH HHS ; NEI NIH HHS ; NHLBI NIH HHS ; NIAID NIH HHS ; NIAMS NIH HHS ; NIA NIH HHS ; NIDDK NIH HHS ; NIGMS NIH HHS ; NINDS NIH HHS ; BLRD VA ; Pancreatic Cancer UK
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/122008]
专题	中国科学院合肥物质科学研究院
通讯作者	Klionsky, Daniel J.
作者单位	1.Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa, Japan 2.Koc Univ, Sch Med, Dept Mol Biol & Genet, Istanbul, Turkey 3.Koc Univ, KUTTAM Res Ctr Translat Med, Sch Med, Istanbul, Turkey 4.Univ Crete, Sch Med, Lab Clin Microbiol & Microbial Pathogenesis, Iraklion, Crete, Greece 5.Fdn Res & Technol, Inst Mol Biol & Biotechnol IMBB, Iraklion, Crete, Greece 6.Inst Canc Res, Canc Therapeut Unit, Sutton, Surrey, England 7.Gulf Med Univ, Coll Pharm, Pharmaceut Sci Dept, Ajman, U Arab Emirates 8.Univ Sydney, Dept Pathol, Sydney, NSW, Australia 9.Univ Sydney, Bosch Inst, Sydney, NSW, Australia 10.Insight Inst Neurosurg & Neurosci, Dept Res, Flint, MI USA
推荐引用方式 GB/T 7714	Klionsky, Daniel J.,Abdel-Aziz, Amal Kamal,Abdelfatah, Sara,et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)[J]. AUTOPHAGY,2021,17.
APA	Klionsky, Daniel J..,Abdel-Aziz, Amal Kamal.,Abdelfatah, Sara.,Abdellatif, Mahmoud.,Abdoli, Asghar.,...&Hu, Ming Chang.(2021).Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).AUTOPHAGY,17.
MLA	Klionsky, Daniel J.,et al."Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)".AUTOPHAGY 17(2021).