In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori

doi:10.1038/s41467-021-22286-x

	In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori
	Zhang, Lufeng 2; Zhang, Liang 2; Deng, Hui 2; Li, Huan 1; Tang, Wentao 2; Guan, Luyao 2; Qiu, Ye 2; Donovan, Michael J.2; Chen, Zhuo 2; Tan, Weihong 2,3
刊名	NATURE COMMUNICATIONS
	2021-03-31
卷号	12
ISSN号	2041-1723
DOI	10.1038/s41467-021-22286-x
通讯作者	Chen, Zhuo(zhuochen@hnu.edu.cn)
英文摘要	Helicobacter pylori infection is a major etiological factor in gastric diseases. However, clinical antibiotic therapy for H. pylori is limited by continuously decreased therapeutic efficacy and side effects to symbiotic bacteria. Herein, we develop an in vivo activatable pH-responsive graphitic nanozyme, PtCo@Graphene (PtCo@G), for selective treatment of H. pylori. Such nanozymes can resist gastric acid corrosion, exhibit oxidase-like activity to stably generate reactive oxygen species only in acidic gastric milieu and demonstrate superior selective bactericidal property. C-18-PEG(n)-Benzeneboronic acid molecules are modified on PtCo@G, improving its targeting capability. Under acidic gastric pH, graphitic nanozymes show notable bactericidal activity toward H. pylori, while no bacterial killing is observed under intestinal conditions. In mouse model, high antibacterial capability toward H. pylori and negligible side effects toward normal tissues and symbiotic bacteria are achieved. Graphitic nanozyme displays the desired enzyme-like activities at corresponding physiological sites and may address critical issues in clinical treatment of H. pylori infections.Helicobacter pylori is a major cause of gastric diseases, but the standard therapy is limited by continuously decreased therapeutic efficacy and side effects to symbiotic bacteria. Here, the authors develop a pH-responsive graphitic nanozyme that is active under low pH gastric conditions, but inactive in intestines, for selective treatment of H. pylori infections.
资助项目	National Natural Science Foundation of China[21522501] ; National Natural Science Foundation of China[21521063] ; Hunan Provincial Natural Science Foundation of China[2018JJ1007] ; Science and Technology Development Fund of Macao S.A.R. (FDCT)[196/2017/A3]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000636902900005
资助机构	National Natural Science Foundation of China ; Hunan Provincial Natural Science Foundation of China ; Science and Technology Development Fund of Macao S.A.R. (FDCT)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/121563]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Zhuo
作者单位	1.Cent South Univ, Xiangya Hosp 3, Dept Gastroenterol, Changsha, Peoples R China 2.Hunan Univ, Hunan Prov Key Lab Biomacromol Chem Biol, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab,Coll Chem & Chem Engn,Coll B, Changsha, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Canc Hosp, Inst Basic Med & Canc IBMC, Hangzhou, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lufeng,Zhang, Liang,Deng, Hui,et al. In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori[J]. NATURE COMMUNICATIONS,2021,12.
APA	Zhang, Lufeng.,Zhang, Liang.,Deng, Hui.,Li, Huan.,Tang, Wentao.,...&Tan, Weihong.(2021).In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori.NATURE COMMUNICATIONS,12.
MLA	Zhang, Lufeng,et al."In vivo activation of pH-responsive oxidase-like graphitic nanozymes for selective killing of Helicobacter pylori".NATURE COMMUNICATIONS 12(2021).