Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe

doi:10.1016/j.snb.2021.129541

	Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe
	Yang, Linlin 1,2; Wang, Jianping 3; Liu, Bianhua 2; Han, Guangmei 4; Wang, Hong 4; Yang, Liang 2; Zhao, Jun 2; Han, Ming-Yong 2; Zhang, Zhongping 2,4
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2021-04-15
卷号	333
关键词	Lipid droplet Solvatochromic fluorescent probe Dynamics Discrimination Cancer cells
DOI	10.1016/j.snb.2021.129541
通讯作者	Zhao, Jun(zjun@iim.ac.cn)
英文摘要	High lipid metabolism is one of the fundamental characteristics of cancer cells different from normal cells due to lots of energy needed for the fast proliferation of cancer cells. As an important organelle to supply cellular energy, however, the difference of lipid droplets (LDs) in their dynamic features between cancer cells and normal cells remains unclear. A key difficulty is to develop a specific fluorescence-tunable probe with high signal-tonoise for rapidly and accurately identifying cancer cells via tracking lipid droplets' dynamics. Here, we report a solvatochromic fluorescent probe, dansyl-morpholine (DNS-M), with high LDs-specificity to achieve the effective discrimination of cancer cells from normal cells by visually profiling the feature difference of LDs dynamics. Probe DNS-M is synthesized easily by one-step reaction, and possesses a large Stokes shift similar to 200 nm, excellent photostablity and LDs-targeted capability with high overlap coefficient up to 0.97. Due to the remarkable biomembrance penetration, LDs-specificity and photostability, DNS-M has been applied to distinguish cancer cells from normal cells rapidly within 1 min in living cells and fixed cells, and real-time track dynamic behaviors of LDs in cancer cells, such as association, dissociation and fusion. More importantly, a significant difference in LDs behavior modes between cancer cells and normal cells is successfully visualized for the first time with our probe. We envision that our probe will become a rapid and easy-to-operate tool for cancer diagnosis and deeper understanding of LDs-related behaviors in cancer cells.
资助项目	National Natural Science Foundation of China[61705239] ; National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21806168] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21976183] ; Natural Science Foundation of Anhui Province[2008085QB69] ; Anhui Provincial Key Research and Development Plan[202004d07020013] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-ZDTP-080] ; Scientific Research Foundation for High-Level Talents of West Anhui University[WGKQ2021001]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000624965300007
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province ; Anhui Provincial Key Research and Development Plan ; Science and Technology Service Network Initiative of Chinese Academy of Sciences ; Scientific Research Foundation for High-Level Talents of West Anhui University
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/121110]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhao, Jun
作者单位	1.West Anhui Univ, Sch Mat & Chem Engn, Key Lab Biomimet Sensor & Detecting Technol Anhui, Luan 237012, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Solid State Phys, Hefei 230031, Anhui, Peoples R China 3.China Jiliang Univ, Coll Life Sci, Zhejiang Prov Key Lab Biometrol & Inspect Quarant, Hangzhou 310018, Peoples R China 4.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Yang, Linlin,Wang, Jianping,Liu, Bianhua,et al. Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,333.
APA	Yang, Linlin.,Wang, Jianping.,Liu, Bianhua.,Han, Guangmei.,Wang, Hong.,...&Zhang, Zhongping.(2021).Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe.SENSORS AND ACTUATORS B-CHEMICAL,333.
MLA	Yang, Linlin,et al."Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe".SENSORS AND ACTUATORS B-CHEMICAL 333(2021).