Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe

doi:10.1016/j.bios.2021.113036

	Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe
	Zhu, Tong 1,2; Yang, Guanqing 3; Liu, Xiaolu 2; Xiang, Pan 2; Yang, Zhenghui 4,5; Zhang, Sijing 3; Chen, Juan 3; Wang, Hong 3; de Souza, Senio Campos 6; Zhang, Zhongping 4,5,7
刊名	BIOSENSORS & BIOELECTRONICS
	2021-04-15
卷号	178
关键词	Mitochondrial ultrastructure 3-Dimensional dynamic Oxidative phosphorylation BODIPY-Pyridine derivative STED nanoscopy
ISSN号	0956-5663
DOI	10.1016/j.bios.2021.113036
通讯作者	Tian, Xiaohe(xiaohe.t@ahu.edu.cn)
英文摘要	Recent advancements in super-resolution nanoscopy allowed the study of mitochondrial biology at nanoscale and boosted the understanding its correlated cellular processes those were previously poorly understood. Nevertheless, studying mitochondrial ultrastructure remains a challenge due to the lack of probes that could target specific mitochondrial substances (e.g. cristae or mtDNA) and survive under harsh super-resolution optical conditions. Herein, in this work, we have rationally constructed a pyridine-BODIPY (Py-BODIPY) derivative that could target mitochondrial membrane in living cells without interfering its physiological microenvironments. Furthermore, we found Py-BODIPY is a membrane potential independent probe, hence it is not limit to live-cell staining but also showed a strong internalization into pre-fixed and stimulus disrupted sample. Importantly, its cristae specificity and superb photostability allow the observation of mitochondrial dynamic nano-structures with an unprecedented resolution, allow demonstrating how mitochondrial 3D ultrastructure evolved under oxidative phosphorylation condition.
资助项目	National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[21871003] ; National Natural Science Foundation of China[21701160] ; Doctor Start-up Fund[S020118002/026] ; Hefei National Laboratory for Physical Sciences at the Microscale[KF2019002] ; Anhui University[S020118002/026] ; Anhui University[S020118002/073] ; Natural Science Foundation of Anhui Province of China[1908085MB30] ; Natural Science Foundation of Anhui Province of China[2008085QB52] ; Open Fund For Discipline Construction from Institute of Physical Science and Information Technology, Anhui University
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000621207600005
资助机构	National Natural Science Foundation of China ; Doctor Start-up Fund ; Hefei National Laboratory for Physical Sciences at the Microscale ; Anhui University ; Natural Science Foundation of Anhui Province of China ; Open Fund For Discipline Construction from Institute of Physical Science and Information Technology, Anhui University
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/120798]
专题	中国科学院合肥物质科学研究院
通讯作者	Tian, Xiaohe
作者单位	1.Sichuan Univ, Huaxi MR Res Ctr HMRRC, Dept Radiol, Funct & Mol Imaging Key Lab Sichuan Prov,West Chi, Chengdu 610041, Peoples R China 2.Anhui Univ, Sch Life Sci, Hefei 230601, Peoples R China 3.Anhui Univ, Dept Chem, Key Lab Funct Inorgan Mat Chem Anhui Prov, Hefei 230601, Peoples R China 4.Anhui Univ, Inst Phys Sci, Hefei 230601, Peoples R China 5.Anhui Univ, Inst Informat Technol, Hefei 230601, Peoples R China 6.UCL, Dept Chem, Gower St, London WC1E 6BT, England 7.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Tong,Yang, Guanqing,Liu, Xiaolu,et al. Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe[J]. BIOSENSORS & BIOELECTRONICS,2021,178.
APA	Zhu, Tong.,Yang, Guanqing.,Liu, Xiaolu.,Xiang, Pan.,Yang, Zhenghui.,...&Tian, Xiaohe.(2021).Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe.BIOSENSORS & BIOELECTRONICS,178.
MLA	Zhu, Tong,et al."Live cell mitochondrial 3-dimensional dynamic ultrastructures under oxidative phosphorylation revealed by a Pyridine-BODIPY probe".BIOSENSORS & BIOELECTRONICS 178(2021).