Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota | |
Liu, Ying1,2,3; Wang, Tong1,2,3; Si, Bo2,3; Du, Hua2,3; Liu, Yun2,3; Waqas, Ahmed2,3; Huang, Shengwei2,3; Zhao, Guoping2,3; Chen, Shaopeng2,3; Xu, An2,3,4 | |
刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY |
2021-03-01 | |
卷号 | 210 |
关键词 | Diesel PM2.5 Intratracheal instillation Gut microbiota Structure Composition |
ISSN号 | 0147-6513 |
DOI | 10.1016/j.ecoenv.2021.111903 |
通讯作者 | Xu, An(anxu@ipp.ac.cu) |
英文摘要 | A diverse and large community of gut microbiota reside in the intestinal tract of various organisms and play important roles in metabolism and immune homeostasis of its host. The disorders of microbiota-host interaction have been closely associated with numerous chronic inflammatory and metabolic diseases, including inflammatory bowel disease and type 2 diabetes. The accumulating evidence has shown that fine particulate matter (PM2.5) exposure contributes to the diabetes, atherosclerosis and inflammatory bowel diseases; however, few studies have explored the impact of inhaled diesel PM2.5 on gut microbiota in vivo. In this study, C57BL/6J mice were exposed to diesel PM2.5 for 14 days via intratracheal instillation, and colon tissues and feces were harvested for microbiota analysis. Using high-throughput sequencing technology, we observed that intratracheally instillated diesel PM2.5 significantly altered the gut microbiota diversity and community. At the phylum and genus levels, principal coordinate analysis (PCoA) and principal component analysis (PCA) indicated pronounced segregation of microbiota compositions, which were further confirmed by beta diversity analysis. As the most affected phylum, Bacteroidetes was greatly diminished by diesel PM2.5. On the genus level, Escherichia, Parabacteroides, Akkermansia, and Oscillibacter were significantly elevated by diesel PM2.5 exposure. Our findings provided clear evidence that exposure to diesel PM2.5 via intratracheal instillation deteriorated the gastrointestinal (GI) tract and significantly altered the structure and composition of gut microbiota, which might subsequently contribute to the developmental abnormalities of inflammation, immunity and metabolism. |
资助项目 | National Natural Science Foundation of China[91743106] |
WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
语种 | 英语 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
WOS记录号 | WOS:000611820900001 |
资助机构 | National Natural Science Foundation of China |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/120179] |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Xu, An |
作者单位 | 1.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 3.Chinese Acad Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, High Field Magnet Lab, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 4.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Ying,Wang, Tong,Si, Bo,et al. Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,210. |
APA | Liu, Ying.,Wang, Tong.,Si, Bo.,Du, Hua.,Liu, Yun.,...&Xu, An.(2021).Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,210. |
MLA | Liu, Ying,et al."Intratracheally instillated diesel PM2.5 significantly altered the structure and composition of indigenous murine gut microbiota".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 210(2021). |
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