LncRNA TUG1 Promotes Growth and Metastasis of Cholangiocarcinoma Cells by Inhibiting miR-29a

doi:10.2147/CMAR.S270515

	LncRNA TUG1 Promotes Growth and Metastasis of Cholangiocarcinoma Cells by Inhibiting miR-29a
	Hao, Wei Yuan; Guo, Li Wen; Luo, Jun; Shao, Guo Liang; Zheng, Jia Ping
刊名	CANCER MANAGEMENT AND RESEARCH
	2020
卷号	12
关键词	LncRNA TUG1 miR-29a cholangiocarcinoma proliferation invasion apoptosis
ISSN号	1179-1322
DOI	10.2147/CMAR.S270515
通讯作者	Zheng, Jia Ping(yuzhengdijiao5369@163.com)
英文摘要	Background: As a highly malignant tumor, cholangiocarcinoma poses a serious threat to human life and health, so exploring the mechanisms of its development and progression at a molecular level is of great significance to the diagnosis and treatment of the disease. Objective: This study was aimed at investigating the effects and related mechanisms of LncRNA TUG1 on cholangiocarcinoma cells. Methods: Cholangiocarcinoma tissues and adjacent tissues (n=82 each), human cholangiocarcinoma cell lines (RBE, QBC939, HuH28), and a human normal biliary epithelial cell line (HIBE) were collected. miR-29a-mimics, miR-29a-inhibitor, miR-NC, si-TUG1, pcDNA3.1 TUG1, and NC were transfected into the cholangiocarcinoma cells. qRT-PCR was performed to detect TUG1 and miR-29a expression in the cholangiocarcinoma tissues and cells. Western blotting (WB) was conducted to detect the expression of Bax, Caspase-3, and Bcl-2 in the cells. CCK-8 assay, Transwell, and flow cytometry were carried out to detect cell proliferation, invasion, and apoptosis. Dual luciferase reporter gene assay (DLRGA) was performed to confirm the correlation of TUG1 with miR-29a. Results: TUG1 was highly expressed while miR-29a was poorly expressed in cholangiocarcinoma cells. TUG1 expression was negatively correlated with miR-29a expression, and TUG1 had a relatively high diagnostic value for cholangiocarcinoma. Cell experiments showed that inhibiting TUG1 expression or up-regulating miR-29a expression could inhibit cholangiocarcinoma cells from proliferation and invasion, and promote their apoptosis, while up-regulating TUG1 or inhibiting miR-29a could promote the proliferation and invasion but inhibit the apoptosis. Rescue experiment showed that overexpressing miR-29a could reverse the effects of high TUG1 expression on cholangiocarcinoma cells. DLRGA confirmed that there was a regulatory relationship between TUG1 and miR-29a. Conclusion: TUG1 is highly expressed in cholangiocarcinoma tissues. It can promote the growth and metastasis of cholangiocarcinoma cells by inhibiting miR-29a, so it may be a new target for diagnosing and treating cholangiocarcinoma.
WOS关键词	SURVIVAL ; LONG ; MIGRATION ; INVASION
WOS研究方向	Oncology
语种	英语
出版者	DOVE MEDICAL PRESS LTD
WOS记录号	WOS:000584026900001
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/105265]
专题	中国科学院合肥物质科学研究院
通讯作者	Zheng, Jia Ping
作者单位	Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Hao, Wei Yuan,Guo, Li Wen,Luo, Jun,et al. LncRNA TUG1 Promotes Growth and Metastasis of Cholangiocarcinoma Cells by Inhibiting miR-29a[J]. CANCER MANAGEMENT AND RESEARCH,2020,12.
APA	Hao, Wei Yuan,Guo, Li Wen,Luo, Jun,Shao, Guo Liang,&Zheng, Jia Ping.(2020).LncRNA TUG1 Promotes Growth and Metastasis of Cholangiocarcinoma Cells by Inhibiting miR-29a.CANCER MANAGEMENT AND RESEARCH,12.
MLA	Hao, Wei Yuan,et al."LncRNA TUG1 Promotes Growth and Metastasis of Cholangiocarcinoma Cells by Inhibiting miR-29a".CANCER MANAGEMENT AND RESEARCH 12(2020).