Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway

doi:10.1089/cbr.2020.3709

	Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway
	Zhou, Qinfei; Sun, Yan
刊名	CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
	2020-08-19
关键词	ABHD5 ATGL circ_cMras LAC NF-κ B signaling pathway p53
ISSN号	1084-9785
DOI	10.1089/cbr.2020.3709
通讯作者	Sun, Yan(nafzbr@163.com)
英文摘要	Background: Lung adenocarcinoma (LAC) is a common malignancy worldwide. Emerging findings indicated that circular RNAs possess complex capacities of gene modulation in tumorigenesis and metastasis. Nevertheless, the role of circular RNA in LAC is still largely unknown. Methods: The level of circular RNA cMras (circ_cMras), alpha-beta hydrolase domain 5 (ABHD5), and adipose triglyceride lipase (ATGL) was determined by quantitative real-time polymerase chain reaction assay. Protein levels of ABHD5, ATGL, p53, p65, and phospho-p65 (p-p65) were examined by western blot. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect cell proliferation in vitro. Cell apoptosis was estimated using flow cytometry. Transwell assay was used to measure cell migration and invasion in A549 and HCC827 cells. Finally, the role of circ_cMras was explored using xenograft tumor model. Results: Low levels of circ_cMras, ABHD5, and ATGL were observed in LAC tissues and cells. Upregulation of circ_cMras could hamper tumor aggression in vitro and in vivo, exhibiting as the inhibition of cell proliferation, migration, invasion, and promotion of cell apoptosis, as well as the inhibition on tumor growth in vivo. Moreover, ABHD5 deletion could overturn the effects of circ_cMras overexpression on cell behaviors in LAC cells. Furthermore, the inhibiting effects of ABHD5 on cell aggression were reversed by ATGL deficiency in vitro. Mechanically, circ_cMras/ABHD5/ATGL axis exerted its role through NF-kappa B signaling pathway in LAC cells. Conclusion: Circ_cMras exerted its function through ABHD5/ATGL axis using NF-kappa B signaling pathway in LAC, which might provide a novel insight for the diagnosis and prognosis of LAC.
WOS关键词	CANCER STATISTICS ; EXPRESSION ; LIPOLYSIS
WOS研究方向	Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	MARY ANN LIEBERT, INC
WOS记录号	WOS:000586228300004
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/105256]
专题	中国科学院合肥物质科学研究院
通讯作者	Sun, Yan
作者单位	Univ Chinese Acad Sci, Dept Comprehens Med Oncol, Canc Hosp, 1 Banshan East Rd, Hangzhou 310011, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Qinfei,Sun, Yan. Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2020.
APA	Zhou, Qinfei,&Sun, Yan.(2020).Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS.
MLA	Zhou, Qinfei,et al."Circular RNA cMras Suppresses the Progression of Lung Adenocarcinoma Through ABHD5/ATGL Axis Using NF-kappa B Signaling Pathway".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS (2020).