Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells

doi:10.1021/acsami.0c07004

	Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells
	Chen, Xigao 1,2,3,4; Qiu, Liping 5; Cai, Ren 1,2,3,4; Cui, Cheng 1,2,3,4; Li, Long 1,2,3,4; Jiang, Jian-hui 5; Tan, Weihong 1,2,3,4,5,6,7
刊名	ACS APPLIED MATERIALS & INTERFACES
	2020-08-26
卷号	12
关键词	aptamer-directed modification living cell membrane protein multiple labeling protein modification bioorthogonal reactions
ISSN号	1944-8244
DOI	10.1021/acsami.0c07004
通讯作者	Jiang, Jian-hui(jianhuijiang@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
英文摘要	Understanding how a cell membrane protein functions on living cells remains a challenge for cell biology. Specific placement of functional molecules on specific proteins in their native environment would allow comprehensive study of proteins' dynamic functions. Existing methods cannot facilely achieve multiple modifications on specific membrane proteins. In this report, we describe an aptamer-induced, protein-specific bio-orthogonal modification technology for precise nongenetic immobilization of multiple small functional molecules on target membrane glycoproteins by combining metabolic technology and aptamer targeting. In brief, DNA probes were designed by modifying aptamers, which bind to target proteins on the surfaces of living cells pretreated with N-azidoacetylmannosamine-tetraacy-lated (Ac(4)ManNAz). The cyclooctynes tagged of DNA probes will approach the azide groups to trigger the bio-orthogonal reactions. After UV irradiation and hybridization with cDNA (complementary DNA), the aptamers can be removed, and the process can be repeated to achieve multiple modifications for multicolor imaging and cell surface nanoengineering on specific proteins.
资助项目	National Institute of General Medical Sciences[GM079359] ; National Cancer Institute[CA133086] ; National Key Scientific Program of China[2011CB911000] ; National Natural Science Foundation of China[21221003] ; National Natural Science Foundation of China[21327009] ; China National Instrumentation Program[2011YQ03012412]
WOS关键词	LIVE CELLS ; ADHESION
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000566662000004
资助机构	National Institute of General Medical Sciences ; National Cancer Institute ; National Key Scientific Program of China ; National Natural Science Foundation of China ; China National Instrumentation Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/104018]
专题	中国科学院合肥物质科学研究院
通讯作者	Jiang, Jian-hui; Tan, Weihong
作者单位	1.Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA 2.Univ Florida, Dept Physiol, Gainesville, FL 32611 USA 3.Univ Florida, Funct Genom, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA 4.Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA 5.Hunan Univ, Aptamer Engn Ctr Hunan Prov, Mol Sci & Biomed Lab MBL,Coll Biol, State Key Lab Chemo Biosensing & Chemometr,Coll C, Changsha 410082, Hunan, Peoples R China 6.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 7.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xigao,Qiu, Liping,Cai, Ren,et al. Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2020,12.
APA	Chen, Xigao.,Qiu, Liping.,Cai, Ren.,Cui, Cheng.,Li, Long.,...&Tan, Weihong.(2020).Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells.ACS APPLIED MATERIALS & INTERFACES,12.
MLA	Chen, Xigao,et al."Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells".ACS APPLIED MATERIALS & INTERFACES 12(2020).