Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS

doi:10.1016/j.jpba.2020.113171

	Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS
	Chen, Jian 2,3,4; Hou, Hongwei 4; Chen, Huan 4; Luo, Yanbo 4; He, Yunlu 4; Wang, Haoyue 2,3,4; Deng, Huimin 4; Zhang, Lirong 6; Zhang, Yunfei 1; Liu, Hansong 5
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2020-05-30
卷号	184
关键词	Pregnanediol-3-glucuronide Serum LC-MS/MS Thyroid cancer Pregnancy
ISSN号	0731-7085
DOI	10.1016/j.jpba.2020.113171
通讯作者	Hou, Hongwei(qsfctc@163.com) ; Wang, An(wangan@aiofm.ac.cn) ; Hu, Qingyuan(huqy1965@163.com)
英文摘要	Pregnanediol-3-glucuronide (PdG) is the major terminal metabolite of progesterone, playing an important role in physiological processes, such as the female menstrual cycle, pregnancy (supports gestation), embryogenesis and maternal immune response of humans and other species. Hence, accurate measurement of PdG in serum/plasma is needed for the evaluation of progesterone production. However, such high-specificity determination of PdG is lacking in clinical sample detection. In this study, a highly sensitive and accurate LC-MS/MS method was firstly established for subsequent measurement of PdG in serum of three different female groups: thyroid cancer patients (TCs), healthy controls (HCs) and pregnant women. The factors affecting the sample preparation, MS/MS method, gradient elution program, selection of chromatographic column and internal standard (IS) have been optimized in this study. Compared with enzyme immunoassay (EIA) method, we used LC-MS/MS to shorten analysis time, increase sensitivity, raise specificity, simplify sample preparation, and reduce costs. As a result, the linear range of the method was from 0.38 to 100 ng/mL with a limit of quantification (LOD) of 0.01 ng/mL. Precision assays showed that relative standard deviation (RSD) was less than 10.6, accuracy was between 90.6 % and 110.4 %, and mean recovery was 103.4 %. In addition, the serum PdG/creatinine levels were significantly down-regulated in TCs and up-regulated in pregnant women versus HCs. Receive operating characteristic curve (ROC) analysis enabled to identify TC with a sensitivity of 83.3 %, specificity of 68.0 % and area under curve (AUC) of 0.781 (95 % CI: 0.684 to 0.879), and it enabled to identify pregnant women with a sensitivity of 94.7 %, specificity of 68.5 % and AUC of 0.811 (95 % CI: 0.732 to 0.890). Our results implied that an increase in female serum PdG/creatinine level might be associated with a risk of pregnancy, but serum PdG/creatinine decreasing might be related to a risk of TC. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[21277174]
WOS关键词	SURVIVORSHIP STATISTICS ; CANCER-TREATMENT ; SEX-HORMONES ; PROGESTERONE ; PAPILLARY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000527297500009
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103341]
专题	中国科学院合肥物质科学研究院
通讯作者	Hou, Hongwei; Wang, An; Hu, Qingyuan
作者单位	1.Zhengzhou Univ, Henan Canc Hosp, Zhengzhou 450008, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Inst Appl Technol, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 4.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou 45000L, Peoples R China 5.Zhengzhou Univ, Zhengzhou Cent Hosp, Zhengzhou 45000L, Peoples R China 6.Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou 450001, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jian,Hou, Hongwei,Chen, Huan,et al. Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2020,184.
APA	Chen, Jian.,Hou, Hongwei.,Chen, Huan.,Luo, Yanbo.,He, Yunlu.,...&Hu, Qingyuan.(2020).Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,184.
MLA	Chen, Jian,et al."Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 184(2020).