Construction of a human cell landscape at single-cell level

doi:10.1038/s41586-020-2157-4

	Construction of a human cell landscape at single-cell level
	Han, Xiaoping 7,17; Zhou, Ziming 7; Fei, Lijiang 7; Sun, Huiyu 7; Wang, Renying 7; Chen, Yao 2; Chen, Haide 3,7; Wang, Jingjing 3,7; Tang, Huanna 4; Ge, Wenhao 5
刊名	NATURE
	2020-05-01
卷号	581
ISSN号	0028-0836
DOI	10.1038/s41586-020-2157-4
通讯作者	Han, Xiaoping(xhan@zju.edu.cn) ; Guo, Guoji(ggj@zju.edu.cn)
英文摘要	Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex systems(1). However, a comprehensive single-cell atlas has not been achieved for humans. Here we use single-cell mRNA sequencing to determine the cell-type composition of all major human organs and construct a scheme for the human cell landscape (HCL). We have uncovered a single-cell hierarchy for many tissues that have not been well characterized. We established a 'single-cell HCL analysis' pipeline that helps to define human cell identity. Finally, we performed a single-cell comparative analysis of landscapes from human and mouse to identify conserved genetic networks. We found that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct. Our results provide a useful resource for the study of human biology. Single-cell RNA sequencing is used to generate a dataset covering all major human organs in both adult and fetal stages, enabling comparison with similar datasets for mouse tissues.
资助项目	National Natural Science Foundation of China[91842301] ; National Natural Science Foundation of China[81770188] ; National Natural Science Foundation of China[31722027] ; National Natural Science Foundation of China[31922049] ; National Natural Science Foundation of China[31701290] ; National Natural Science Foundation of China[31871473] ; National Key Research and Development Program[2018YFA0107804] ; National Key Research and Development Program[2018YFA0107801] ; National Key Research and Development Program[2018YFA0800503] ; National Key Research and Development Program[2018YFC1005003] ; Zhejiang Provincial Natural Science Foundation of China[R17H080001] ; Fundamental Research Funds for the Central Universities
WOS关键词	MESSENGER-RNA-SEQ ; REVEALS ; MOUSE ; EXPRESSION ; IDENTITY ; RECONSTRUCTION ; DIVERSITY ; ATLAS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000532687800001
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program ; Zhejiang Provincial Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103247]
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Xiaoping; Guo, Guoji
作者单位	1.Veritas Genet Asia, Hangzhou, Peoples R China 2.Zhejiang Univ, Womens Hosp, Sch Med, Dept Reprod Endocrinol, Hangzhou, Peoples R China 3.Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Zhejiang Prov Key Lab Tissue Engn & Regenerat Med, Hangzhou, Peoples R China 4.Zhejiang Univ, Affiliated Hosp 1, Kidney Dis Ctr, Sch Med, Hangzhou, Peoples R China 5.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Hepatobiliary & Pancreat Surg, Hangzhou, Peoples R China 6.Annoroad Gene Technol, Beijing, Peoples R China 7.Zhejiang Univ, Ctr Stem Cell & Regenerat Med, Sch Med, Hangzhou, Peoples R China 8.Zhejiang Univ, Coll Life Sci, Hangzhou, Peoples R China 9.Zhejiang Univ, Ctr Neurosci, Sch Med, Hangzhou, Peoples R China 10.Zhejiang Univ, Life Sci Inst, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoping,Zhou, Ziming,Fei, Lijiang,et al. Construction of a human cell landscape at single-cell level[J]. NATURE,2020,581.
APA	Han, Xiaoping.,Zhou, Ziming.,Fei, Lijiang.,Sun, Huiyu.,Wang, Renying.,...&Guo, Guoji.(2020).Construction of a human cell landscape at single-cell level.NATURE,581.
MLA	Han, Xiaoping,et al."Construction of a human cell landscape at single-cell level".NATURE 581(2020).