A nanomicelle with miR-34a and doxorubicin reverses the drug resistance of cisplatin in esophageal carcinoma cells by inhibiting SIRT1 signal pathway

doi:10.21037/tcr-19-975

	A nanomicelle with miR-34a and doxorubicin reverses the drug resistance of cisplatin in esophageal carcinoma cells by inhibiting SIRT1 signal pathway
	Fang, Jun 2,3; Wang, Yuezhen 2,3; Wang, Zhun 2,3; Xie, Tieming 3,4; Yan, Fengqin 2,3,5; Wang, Lei 2,3,4; Hua, Jianfeng 1; Wang, Fangzheng 2,3,5; Fu, Zhenfu 2,3,5; Ye, Zhimin 2,3,5
刊名	TRANSLATIONAL CANCER RESEARCH
	2020-07-01
卷号	9
关键词	Nanomicelle miR-34a esophageal carcinoma (EC) p53 SIRT1 drug resistance
ISSN号	2218-676X
DOI	10.21037/tcr-19-975
通讯作者	Fu, Zhenfu(fuzf1000@163.com) ; Ye, Zhimin(yezm@zjcc.org.cn)
英文摘要	Background: Esophageal carcinoma (EC) is one of the most deadly malignant tumors in the world. Surgery, combined with chemotherapy or radiotherapy, is the traditional strategy for the treatment of EC. Cisplatin (CDDP) is a common chemotherapy drug widely used to treat EC due to its powerful anti-tumor effect. However, CDDP is subject to intrinsic or acquired resistance in EC cells, which badly hinders the efficacy of chemotherapy. The resistance phenomenon is mostly caused by the p53 mutant in the EC and the low efficiency of the drug delivery system. Methods: In this study, a specially designed nanomicelle was used to promote the anti-tumor effect of chemotherapy drugs against the CDDP-resistant EC cells. The nanomicelle consisted of miR-34a, doxorubicin (DOX), polyethylene glycol (PEG), and other excipients in an appropriate ratio. Results: The results showed that the nanomicelle could exert significant cell proliferation inhibition and apoptosis-inducing effects in the CDDP-resistant EC cells. The endogenous expression of miR-34a in the CDDP-resistant EC cells was promoted by the incubation with the nanomicelle. After incubation with the nanomicelle, the expression of protein SIRT1 was inhibited, and the expression of caspase3 was promoted significantly in the CDDP-resistant EC cells. Conclusions: Our results indicate that the specially designed nanomicelle can exert promising anti-tumor effects by introducing miR-34a to inhibit SIRT1 signaling pathway and enhance the efficiency of the drug delivery system.
资助项目	National Natural Science Foundation of China[81502647] ; National Natural Science Foundation of China[81502646] ; Zhejiang Provincial Natural Science Foundation of China[LY17H160039] ; Zhejiang Provincial Natural Science Foundation of China[LY18H160031] ; Zhejiang Provincial Natural Science Foundation of China[LY19H160004] ; Zhejiang Medical and Health Science and Technology Project[2016KYA051] ; Zhejiang Medical and Health Science and Technology Project[2016146960] ; Zhejiang Medical and Health Science and Technology Project[2018RC083] ; Zhejiang Medical and Health Science and Technology Project[2018260661] ; Zhejiang Medical and Health Science and Technology Project[2019328882]
WOS关键词	CANCER-CELLS ; P53 ; CHEMORADIOTHERAPY ; ADENOCARCINOMAS ; SURVIVAL ; DELIVERY ; PROTEIN
WOS研究方向	Oncology
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000553238500011
资助机构	National Natural Science Foundation of China ; Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Medical and Health Science and Technology Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/102917]
专题	中国科学院合肥物质科学研究院
通讯作者	Fu, Zhenfu; Ye, Zhimin
作者单位	1.Quzhou Kecheng Hosp, Dept Radiat Oncol, Quzhou, Peoples R China 2.Univ Chinese Acad Sci, Dept Radiat Oncol, Canc Hosp, Zhejiang Canc Hosp, Banshan East Rd, Hangzhou, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China 4.Univ Chinese Acad Sci, Dept Radiol, Canc Hosp, Zhejiang Canc Hosp, Hangzhou, Peoples R China 5.Key Lab Head & Neck Canc Translat Res Zhejiang Pr, Banshan East Rd, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Fang, Jun,Wang, Yuezhen,Wang, Zhun,et al. A nanomicelle with miR-34a and doxorubicin reverses the drug resistance of cisplatin in esophageal carcinoma cells by inhibiting SIRT1 signal pathway[J]. TRANSLATIONAL CANCER RESEARCH,2020,9.
APA	Fang, Jun.,Wang, Yuezhen.,Wang, Zhun.,Xie, Tieming.,Yan, Fengqin.,...&Ye, Zhimin.(2020).A nanomicelle with miR-34a and doxorubicin reverses the drug resistance of cisplatin in esophageal carcinoma cells by inhibiting SIRT1 signal pathway.TRANSLATIONAL CANCER RESEARCH,9.
MLA	Fang, Jun,et al."A nanomicelle with miR-34a and doxorubicin reverses the drug resistance of cisplatin in esophageal carcinoma cells by inhibiting SIRT1 signal pathway".TRANSLATIONAL CANCER RESEARCH 9(2020).