Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy

doi:10.1007/s00216-020-02710-9

	Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy
	Cao, Chentai 1,2; Li, Pan 1; Liao, Hongmei 3; Wang, Junping 4; Tang, Xianghu 5; Yang, Liangbao 1,4
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2020-07-01
卷号	412
关键词	Dynamic surface-enhanced Raman spectroscopy SERS probe Saxitoxin Electrostatic interaction Hydrogen bond
ISSN号	1618-2642
DOI	10.1007/s00216-020-02710-9
通讯作者	Li, Pan(lipan2011@iim.ac.cn) ; Liao, Hongmei(549828750@qq.com) ; Yang, Liangbao(lbyang@iim.ac.cn)
英文摘要	Saxitoxin (STX) as one of the most harmful and typical paralytic shellfish toxins, is posing a serious threat to environmental and human health, thus it is essential to develop a sensitive and reliable analytical method for STX detection. Herein, we proposed a strategy for rapid and sensitive detection of STX with surface-enhanced Raman spectroscopy (SERS), by employing cysteine modified gold nanoparticles (Cys-AuNPs) as SERS probe to capture STX molecules through electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Moreover, the XPS and zeta potential results indicated that Cys could bond to AuNPs through Au-S bonds and the addition of STX could induce the efficient aggregation of Cys-AuNPs owing to the presence of electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Furthermore, considering the high sensitivity and stability of the dynamic surface-enhanced Raman spectroscopy (D-SERS) strategy with the formation of a 3D hotspot matrix, the highly sensitive detection of STX was realized to a level of 1 x 10(-7) M by using the D-SERS strategy. Consequently, Cys-AuNPs as high affinity substrates can provide high sensitivity for the detection of STX through the D-SERS strategy.
资助项目	National Major Scientific and Technological Special Project for Significant New Drugs Development[2018ZX09J18112] ; National Science Foundation of China[61875206] ; CASHIPS Directors Fund[YZJJ2019QN13] ; Sci-tech Police Project of Anhui Province[806116381020] ; Sci-tech Police Project of Anhui Province[904038287034]
WOS关键词	SENSITIVE DETECTION ; L-CYSTEINE ; LIQUID-CHROMATOGRAPHY ; GOLD NANOPARTICLES ; SAXITOXIN ; SILVER ; STABILITY ; NEOSAXITOXIN ; APTASENSOR ; APTAMER
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000543580600002
资助机构	National Major Scientific and Technological Special Project for Significant New Drugs Development ; National Science Foundation of China ; CASHIPS Directors Fund ; Sci-tech Police Project of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/92839]
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Pan; Liao, Hongmei; Yang, Liangbao
作者单位	1.Chinese Acad Sci, Anhui Prov Key Lab Med Phys & Technol, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.Fuyang Inst Technol, Fuyang 236031, Anhui, Peoples R China 4.Chinese Acad Sci, Hefei Canc Hosp, Dept Pharm, Hefei 230031, Anhui, Peoples R China 5.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Cao, Chentai,Li, Pan,Liao, Hongmei,et al. Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020,412.
APA	Cao, Chentai,Li, Pan,Liao, Hongmei,Wang, Junping,Tang, Xianghu,&Yang, Liangbao.(2020).Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412.
MLA	Cao, Chentai,et al."Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412(2020).