Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering

doi:10.1007/s00604-020-04330-7

	Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering
	Xu, Wei 2,3,4; Zhao, Aiwu 2,3,4; Zuo, Fangtao 2,3,4; Khan, Ranjha 1; Hussain, Hafiz Muhammad Jafar 1; Chang, Jianguo 3,4
刊名	MICROCHIMICA ACTA
	2020-06-12
卷号	187
关键词	Au@DTNB@Ag core-shell nanoparticles Surface-enhanced Raman scattering miRNA-21 Duplex-specific nuclease Human serum
ISSN号	0026-3672
DOI	10.1007/s00604-020-04330-7
通讯作者	Zhao, Aiwu(awzhao@iim.ac.cn)
英文摘要	A novel surface-enhanced Raman scattering (SERS) analysis strategy has been designed combining Au@DTNB@Ag core-shell nanoparticles (DTNB attachment on gold nanoparticles, then encapsulated in Ag shell nanoparticles named as ADANPs) and duplex-specific nuclease signal amplification (DSNSA) platform. Firstly, ADANPs and magnetic substrate of Fe3O4 nanoparticles were covalently attached to the 3 '- and 5 '- end of capture probe (CP) targeting miRNA-21. Upon the addition of target miRNA-21, these heteroduplexes were specifically cleaved by DSN and resulted in ADANPs that were released from the surface of Fe3O4 nanoparticles (Fe3O4 NPs). At the same time, miRNA-21 remained intact and can rehybridize another DNA probe to trigger the signal-amplifying reaction. Based on this principle, the developed SERS method exhibited good linearity in the range 0 to 1 nM for miRNA-21 with a limit of detection (LOD) of 0.084 fM and has an ability to differentiate even a single-base mismatched sequence on the target sequence or other miRNA sequence. The results provide a novel SERS method which can successfully been applied to the miRNA-21 detection in human serum.
资助项目	National Natural Science Foundation of China[61875255] ; Hefei Center of Physical Science Technology[2018ZYFX005]
WOS关键词	QUANTITATIVE DETECTION ; SPECTROSCOPY PLATFORM ; ONE-STEP ; BIOSENSOR ; STRATEGY ; GO
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000542241300002
资助机构	National Natural Science Foundation of China ; Hefei Center of Physical Science Technology
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/71409]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhao, Aiwu
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 2.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,et al. Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering[J]. MICROCHIMICA ACTA,2020,187.
APA	Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,Khan, Ranjha,Hussain, Hafiz Muhammad Jafar,&Chang, Jianguo.(2020).Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering.MICROCHIMICA ACTA,187.
MLA	Xu, Wei,et al."Au@Ag core-shell nanoparticles for microRNA-21 determination based on duplex-specific nuclease signal amplification and surface-enhanced Raman scattering".MICROCHIMICA ACTA 187(2020).