A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

doi:10.1039/d0cc02691c

	A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET
	Shi, Pan 4,5; Zhang, Yanan 4,5; Lv, Pei 4,5; Fang, Wei 4,5; Ling, Shenglong 4,5; Guo, Xiaoqi 4,5; Li, Dong 4,5; Liu, Sanling 4,5; Sun, Demeng 4,5; Zhang, Longhua 4,5
刊名	CHEMICAL COMMUNICATIONS
	2020-06-28
卷号	56
ISSN号	1359-7345
DOI	10.1039/d0cc02691c
通讯作者	Shi, Pan(shipan@ustc.edu.cn) ; Tian, Changlin(cltian@ustc.edu.cn)
英文摘要	The dynamics of GPCRs (G protein-coupled receptors) coupling for cognate G proteins play a critical role in signal transduction. Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differences of G alpha(i1)(inhibitory G proteins) and G alpha(s)(stimulatory G proteins) upon beta(2)AR (beta(2)-adrenergic receptor) coupling. It offers a new generally applicable method to probe protein dynamic interactions or conformational changes.
资助项目	National Key Research and Development Project of China[2016YFA0400903] ; National Key Research and Development Project of China[2017YFA0505201] ; National Key Research and Development Project of China[2017YFA0505400] ; National Natural Science Foundation of China[21825703] ; National Natural Science Foundation of China[31971152]
WOS关键词	ALPHA-SUBUNIT ; ACTIVATION ; NUCLEOTIDE
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000542898200002
资助机构	National Key Research and Development Project of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/71362]
专题	中国科学院合肥物质科学研究院
通讯作者	Shi, Pan; Tian, Changlin
作者单位	1.Univ Sci & Technol China, Anhui Lab Adv Photon Sci & Technol, Hefei 230026, Peoples R China 2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230030, Peoples R China 3.ShanghaiTech Univ, iHuman Inst, 99 HaiKe Rd, Shanghai 201210, Peoples R China 4.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 5.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
推荐引用方式 GB/T 7714	Shi, Pan,Zhang, Yanan,Lv, Pei,et al. A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET[J]. CHEMICAL COMMUNICATIONS,2020,56.
APA	Shi, Pan.,Zhang, Yanan.,Lv, Pei.,Fang, Wei.,Ling, Shenglong.,...&Tian, Changlin.(2020).A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET.CHEMICAL COMMUNICATIONS,56.
MLA	Shi, Pan,et al."A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET".CHEMICAL COMMUNICATIONS 56(2020).