An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model

doi:10.21037/tcr-19-2844

	An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model
	Jiang, Chuner 5,6,7; Pan, Yongming 4; Huang, Junjie 4; He, Xiangming 5,6,7; Ni, Jianfen 5,6,7; Ding, Xiaowen 5,6,7; Zou, Dehong 5,6,7; Wang, Fangzheng 1,2,3
刊名	TRANSLATIONAL CANCER RESEARCH
	2020-08-01
卷号	9
关键词	Autologous adipose tissue dermal outer capsule prosthesis cosmetic effect histopathologic changes
ISSN号	2218-676X
DOI	10.21037/tcr-19-2844
通讯作者	Zou, Dehong(zoudh1964@126.com)
英文摘要	Background: Autologous adipose tissue grafting is a valuable strategy for breast reconstruction in breast cancer patients. However, adipose tissue is absorbed and liquefies easily, and the cosmetic effects do not last. The purpose of this study is to evaluate the cosmetic effects and histopathologic changes of the prostheses produced by inactivated autologous subcutaneous adipose tissue and dermal outer capsule in the mini-pig model. Methods: This prospective study was conducted in 6 Bama adult female mini-pigs weighing 15.5-20 kg. Autologous subcutaneous adipose tissue and dermis were harvested and made into the different prostheses. Then, the prostheses were implanted into abdominal subcutaneous tissue of the mini-pigs. At the 14th and 30th day after implantation, the skin appearances were observed, and the prostheses were harvested. The histopathologic changes of adipocytes and dermis in the different prostheses were evaluated. Results: In vitro experiments showed that there were no significant changes in the size and histomorphology of autologous adipose tissue and/or dermis in the different prostheses. However, the results of the in vivo experiment found that the prosthesis produced by the inactivated adipose tissue with a dermal outer capsule produced the best cosmetic effect and the least fibrous connective tissue proliferation around the prosthesis. Moreover, the fewest proliferative fibrous connective tissues and infiltrated inflammatory cells were found in the adipocytes wrapped by dermis. The adipocyte morphology was normal. Conclusions: Results in this animal model indicated that the prosthesis produced by inactivated autologous subcutaneous adipose tissue with a dermal outer capsule may be a promising prosthesis for breast reconstruction. Validation of this new prosthesis requires more experiments to assess the long-term cosmetic effects and histopathologic changes.
资助项目	Medical and Health Science and Technology Program of Zhejiang Province[2020PY037] ; Medical and Health Science and Technology Program of Zhejiang Province[2020KY084] ; Medical and Health Science and Technology Program of Zhejiang Province[2019KY041] ; Medical and Health Science and Technology Program of Zhejiang Province[2011RCA014] ; Medical and Health Science and Technology Program of Zhejiang Province[2006A016] ; Medical and Health Science and Technology Program of Zhejiang Province[2005B012] ; National Natural Science Foundation of Zhejiang Province of China[LY15H1800012015] ; National Natural Science Foundation of China[81502646] ; National Natural Science Foundation of China[81502647]
WOS关键词	BREAST-CANCER PATIENTS ; RECONSTRUCTION ; SURGERY ; SAFETY ; STATISTICS
WOS研究方向	Oncology
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000562138400031
资助机构	Medical and Health Science and Technology Program of Zhejiang Province ; National Natural Science Foundation of Zhejiang Province of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/70550]
专题	中国科学院合肥物质科学研究院
通讯作者	Zou, Dehong
作者单位	1.Zhejiang Key Lab Radiat Oncol, Hangzhou, Peoples R China 2.Zhejiang Canc Hosp, Dept Radiat Oncol, Hangzhou, Peoples R China 3.Univ Chinese Acad Sci, Canc Hosp, Dept Radiat Oncol, Hangzhou, Peoples R China 4.Zhejiang Univ Tradit Chinese Med, Anim Expt Res Ctr, Hangzhou, Peoples R China 5.Training Base Standardized Treatment Lymphedema, Hangzhou, Peoples R China 6.Zhejiang Canc Hosp, Dept Breast Surg, Hangzhou, Peoples R China 7.Univ Chinese Acad Sci, Canc Hosp, Dept Breast Surg, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Chuner,Pan, Yongming,Huang, Junjie,et al. An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model[J]. TRANSLATIONAL CANCER RESEARCH,2020,9.
APA	Jiang, Chuner.,Pan, Yongming.,Huang, Junjie.,He, Xiangming.,Ni, Jianfen.,...&Wang, Fangzheng.(2020).An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model.TRANSLATIONAL CANCER RESEARCH,9.
MLA	Jiang, Chuner,et al."An experimental study on the prosthesis produced by inactivated autologous subcutaneous adipose tissue and skin dermal tissue in a mini-pig model".TRANSLATIONAL CANCER RESEARCH 9(2020).