Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells

	Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2015
卷号	42
关键词	EPITHELIAL-MESENCHYMAL TRANSITION STEM-CELLS METASTASIS
ISSN号	1000-3282
其他题名	Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells
英文摘要	The incidence rate of epithelial ovarian cancers ranks third among female reproductive system cancers, and its mortality rate stands first among the list of gynecologic tumors.The standard mode of treatment is cytoreductive surgery(debulking)plus the administration of platinum-based chemotherapy drugs.About 70% of advanced patients who receive standard treatments can achieve clinical remission.However, a certain percentage of patients with clinical remission will still experience tumor recurrence, metastasis, and drug resistance.Thus, an exploration on the common interrelated molecular mechanisms of epithelial ovarian cancer metastasis and drug resistance is the focus of our study.Epithelial-mesenchymal transition(EMT)is not only the basis of biological processes of embryonic development, but it also can lead to the enhancement of invasion and the metastasis ability of some epithelial tumor cells.At the same time, it can reduce the sensitivity of tumor cells to chemotherapy drugs.RAC1, which is one of the members of the Rho subfamily, can mediate important signal transductions via an active form of GTP-RAC1, and play a role as a molecular switch in the regulation of cell biological behaviors, such as EMT.And based on a large amount of basic research, RAC1, as one of the member of the Rho subfamily appears to play significant role in the proliferation, differentiation, apoptosis, movement, invasion, and transfer of various tumor cells as well as blood vessels.Therefore, this study focuses on the occurrence process of EMT in SKOV3 cells, which is mediated by RAC1activation, as well as the relationship between the process of cell sensitivity and cisplatin(CDDP), and the ability of invasion and metastasis.The Boyden chamber method was used to compare the migration and invasion ability between two kinds of cells, and the results showed that the adhesion and invasion abilities of SKOV3/DDP were significantly increased(Figure 1a, b).Meanwhile, we compared the expression level of GTP-RAC1between two kinds of cells(SKOV3 and SKOV3/ DDP).The results showed that the expression level of GTP-RAC1in SKOV3/DDP was apparently higher than that in SKOV3 cells(Figure 1c).We compared the expression levels of EMT-related key proteins, such as E-cadherin(CDH1), Claudin(CLDN1), Vimentin(VIM), Snail1(SNAI1)and Slug(SNAI2)cells, between two groups of cells using RT-PCR and Western blot methods.We found that in SKOV3/DDP cells, the expression levels of VIM and SNAI1were increased, and the expression levels of CDH1and CLDN1were decreased(Figure 1d, e).
语种	英语
CSCD记录号	CSCD:5356701
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/48648]
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2015,42.
APA	(2015).Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,42.
MLA	"Chemoresistance to CDDP Induces EMT Through Activation of RAC1 in SKOV3 Human Ovarian Cancer Cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 42(2015).