Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects

	Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects
	Zhang, Xinxin; Wang, Xingbin; Khurm, Muhammad; Zhan, Guanqun; Zhang, Hui; Ito, Yoichiro; Guo, Zengjun
刊名	JOURNAL OF PROTEOME RESEARCH
	2020
卷号	19 期号:3
关键词	diosgenin cerebral ischemic reperfusion anti-inflammatory STAT2 HIKESHI
英文摘要	Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in the pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment remain elusive. In the present study, the proteomics analysis of the brain tissues from I/R rats after DIO treatment was performed using an integrated TMT-based quantitative proteomic approach coupled with the liquid chromatography with tandem mass spectrometry technology. A total of 5043 proteins (ProteomeXchange identifier: PXD016303) were identified, of which 58 common differentially expressed proteins were significantly dysregulated in comparison between sham versus I/R and I/R versus DIO. The eight validated proteins including EPG5, STAT2, CPT1A, EIF2AK2, GGCT, HIKESHI, TNFAIP8, and EMC6 by quantitative polymerase chain reaction and western blotting consistently supported the TMT-based proteomic results, which were mainly associated with autophagy and inflammation response. Considering the anti-inflammatory characters of DIO, the biological functions of STAT2 and HIKESHI that are the probable direct anti-inflammatory targets were further investigated during the course of I/R treated with DIO. In addition, the combination of verified STAT2 and HIKESHI in peripheral blood samples from stroke patients resulted in the area under the curve value of 0.765 with P < 0.004 to distinguish stroke patients from healthy controls. Taken together, the current findings first mapped comprehensive proteomic changes after I/R was treated with DIO to better decipher the molecular mechanisms mainly based on the anti-inflammatory aspect underlying this therapeutic effect, providing a foundation for developing potentially therapeutic targets of anti-I/R of DIO and clinically prognostic biomarkers of stroke.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60396]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Xinxin,Wang, Xingbin,Khurm, Muhammad,et al. Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(3).
APA	Zhang, Xinxin.,Wang, Xingbin.,Khurm, Muhammad.,Zhan, Guanqun.,Zhang, Hui.,...&Guo, Zengjun.(2020).Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects.JOURNAL OF PROTEOME RESEARCH,19(3).
MLA	Zhang, Xinxin,et al."Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects".JOURNAL OF PROTEOME RESEARCH 19.3(2020).