Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening

	Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening
	Fang, Yi; Zhao, Chengzhen; Xiang, Hai; Jia, GongXue; Zhong, Rongzhen
刊名	REPRODUCTION IN DOMESTIC ANIMALS
	2020
卷号	55 期号:9
关键词	cryopreservation melatonin mitochondrial permeability transition pore ram sperm
英文摘要	Cryopreservation damages permeability of sperm mitochondrial membranes, with formation of a mitochondrial permeability transition pore (mPTP). Mitochondria are both a primary synthesis site and principle target for melatonin, which can directly inhibit mPTP formation. The objective was to determine effects of melatonin on mPTP opening of frozen-thawed ram sperm and elucidate underlying pathways by antagonist and agonists of melatonin receptors (MTs), and antagonists of PI3K and GSK 3 beta treatments; furthermore, plasma membrane integrity, mitochondrial membrane potential (Delta psi m), mitochondrial cytochrome c (Cyt c) release and fertilization were analysed to assess the effect of mPTP status mediated by melatonin on quality of frozen-thawed sperm. Fresh ram semen was diluted in glucose-egg yolk buffer with 0 or 10(-7) M melatonin (frozen and frozen + melatonin groups, respectively) and slow-frozen. In frozen-thawed sperm, melatonin added at initiation of 4 degrees C equilibration was most effective for inhibiting mPTP opening, decreasing peptidyl-prolyl-cis/trans isomerase activity of cyclophilin D and increasing plasma membrane integrity, Delta psi m, mitochondrial Cyt c concentration and fertilizing ability (p .05). In a mechanistic study, the melatonin receptor (MT)1 antagonist eliminated inhibition of melatonin on mPTP opening, whereas MT1 agonist had opposite effects (p < .05). Neither MT2 antagonist nor agonist had significant effect, but PI3K and/or GSK 3 beta antagonist decreased inhibition of MT1 agonist on mPTP opening (p .05). In conclusion, melatonin improved sperm cryopreservation, perhaps by acting on MT1 via the PI3K-Akt-GSK 3 beta pathway to inhibit mPTP opening.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60280]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fang, Yi,Zhao, Chengzhen,Xiang, Hai,et al. Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening[J]. REPRODUCTION IN DOMESTIC ANIMALS,2020,55(9).
APA	Fang, Yi,Zhao, Chengzhen,Xiang, Hai,Jia, GongXue,&Zhong, Rongzhen.(2020).Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening.REPRODUCTION IN DOMESTIC ANIMALS,55(9).
MLA	Fang, Yi,et al."Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening".REPRODUCTION IN DOMESTIC ANIMALS 55.9(2020).