Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis | |
Yun JL5; Zheng XW5; Xu P1,5; Zheng X(郑旭)4; Xu JY3,5; Cao C2,6; Fu YS2; Xu BX3,5; Dai X5; Wang Y5 | |
刊名 | SMALL |
2020-03 | |
页码 | 8 |
关键词 | droplet fluorescence-activated cell sorting microfluidics single-cell analysis single-cell sequencing |
ISSN号 | 1613-6810 |
DOI | 10.1002/smll.201903739 |
英文摘要 | Single-cell analysis offers unprecedented resolution for the investigation of cellular heterogeneity and the capture of rare cells from large populations. Here, described is a simple method named interfacial nanoinjection (INJ), which can miniaturize various single-cell assays to be performed in nanoliter water-in-oil droplets on standard microwell plates. The INJ droplet handler can adjust droplet volumes for multistep reactions on demand with high precision and excellent monodispersity, and consequently enables a wide range of single-cell assays. Importantly, INJ can be coupled with fluorescence-activated cell sorting (FACS), which is currently the most effective and accurate single-cell sorting and isolation method. FACS-INJ pipelines for high-throughput plate well-based single-cell analyses, including single-cell proliferation, drug-resistance testing, polymerase chain reaction (PCR), reverse-transcription PCR, and whole-genome sequencing are introduced. This FACS-INJ pipeline is compatible with a wide range of samples and can be extended to various single-cell analysis applications in microbiology, cell biology, and biomedical diagnostics. |
分类号 | 一类 |
资助项目 | China Ocean Mineral Resources RD Association[DY135-B2-02] ; National Natural Science Foundation of China[41977196] ; National Natural Science Foundation of China[21822408] ; National Natural Science Foundation of China[21525521] ; National Natural Science Foundation of China[11832017] ; National Natural Science Foundation of China[11572335] ; National Key R&D Program of China[2016YFE0205800] ; National Key R&D Program of China[2016YFC0100900] ; National Key R&D Program of China[2018YFC0310703] ; Chinese Academy of Sciences[XDB15040102] ; Chinese Academy of Sciences[QYZDB-SSW-SMC008] ; Chinese Academy of Sciences[QYZDB-SSW-JSC036] ; Chinese Academy of Sciences[KFZD-SW-219-4] ; Chinese Academy of Sciences[XDB22040403] ; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences[SKLMR-20150602] |
WOS关键词 | HIGH-THROUGHPUT ; AMPLIFICATION ; TECHNOLOGIES ; MICROBES ; REVEALS ; GENOMES ; DISPLAY |
WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
语种 | 英语 |
WOS记录号 | WOS:000488077200001 |
资助机构 | China Ocean Mineral Resources RD Association ; National Natural Science Foundation of China ; National Key R&D Program of China ; Chinese Academy of Sciences ; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences |
其他责任者 | Huang, Li |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/80643] |
专题 | 力学研究所_非线性力学国家重点实验室 |
作者单位 | 1.Univ Calif San Francisco, Bioengn & Therapeut Sci, San Francisco, CA 94158 USA; 2.Peking Univ, Biomed Pioneering Innovat Ctr BIOPIC, Beijing Adv Innovat Ctr Genom ICG,Sch Life Sci, Peking Tsinghua Ctr Life Sci,Coll Engn, Beijing 100871, Peoples R China; 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China; 4.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China; 5.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China; 6.Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08540 USA 7.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China; |
推荐引用方式 GB/T 7714 | Yun JL,Zheng XW,Xu P,et al. Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis[J]. SMALL,2020:8. |
APA | Yun JL.,Zheng XW.,Xu P.,Zheng X.,Xu JY.,...&Du WB.(2020).Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis.SMALL,8. |
MLA | Yun JL,et al."Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis".SMALL (2020):8. |
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