Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax

doi:10.1016/j.ijbiomac.2019.06.233

	Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax
	Yin, Ying 1; Yu, Weili 2; Li, Yujie 1; Liu, Kun 1; Zai, Xiaodong 1; Zhang, Jun 1; Fu, Ling 1; Hu, Tao 2; Xu, Junjie 1; Chen, Wei 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-09-15
卷号	137 页码:790-800
关键词	Antigen delivery system Anthrax Self-conjugation Polyethylene glycol
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.06.233
英文摘要	Anthrax is an acute and highly lethal disease caused by Bacillus anthracis. Protective antigen (PA) is the primary candidate antigen for the anthrax vaccines. However, PA suffers from poor immunogenicity with short-term anti PA antibody response. High effectiveness, durable immunity, and minimal risk are required for development of an effective anthrax vaccine. In the present study, PA was self-conjugated by 8-arm polyethylene glycol (PEG) and further by thioester chemistry. As a result, 3-5 PA molecules were covalently conjugated and functioned as an antigen delivery system. The conjugate (PA-PEG) could maintain the structural properties of PA and increase the thermal stability of PA. PA-PEG could elicit a robust anti-PA IgG and neutralization antibody response in the magnitude and quality. The antibodies could be largely maintained for 180 days after three immunizations of PA-PEG. PA-PEG effectively stimulated the maturation of dendritic cell and rapidly induced the germinal center (GC) reaction. The percentages of the GC B-cells and T follicular helper (Tfh) cells were thus significantly augmented. The inflammatory response elicited by PA-PEG was comparable to those by PBS and PA. Therefore, PA-PEG is expected as an effective anthrax vaccine candidate with durable immunoprotection against anthrax. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	National Science and Technology Major Project of China[2016ZX10004001] ; National Natural Science Foundation of China[81703445] ; National Natural Science Foundation of China[81700181] ; National Natural Science Foundation of China[31300760]
WOS关键词	INHALATIONAL ANTHRAX ; DELTA INULIN ; VACCINE ; IMMUNOGENICITY ; PEGYLATION ; IMMUNITY ; CELLS ; ADVAX(TM) ; IMPROVES ; SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000484644100086
资助机构	National Science and Technology Major Project of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/30751]
专题	中国科学院过程工程研究所
通讯作者	Hu, Tao; Xu, Junjie; Chen, Wei
作者单位	1.Beijing Inst Biotechnol, Lab Vaccine & Antibody Engn, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yin, Ying,Yu, Weili,Li, Yujie,et al. Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,137:790-800.
APA	Yin, Ying.,Yu, Weili.,Li, Yujie.,Liu, Kun.,Zai, Xiaodong.,...&Chen, Wei.(2019).Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,137,790-800.
MLA	Yin, Ying,et al."Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 137(2019):790-800.