Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China

doi:10.3390/md7010024

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	Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China
	Hong, Kui 3; Gao, An-Hui 5; Xie, Qing-Yi 3; Gao, Hao 4; Zhuang, Ling 3; Lin, Hai-Peng 3; Yu, Hai-Ping 5; Li, Jia5 ; Yao, Xin-Sheng 4; Goodfellow, Michael 1
刊名	MARINE DRUGS
	2009-03
卷号	7 期号:1 页码:24-44
关键词	Mangroves actinomycete diversity marine drug discovery high-throughput screening growth inhibition protein tyrosine phosphatase 1B aurora kinase A caspase 3
ISSN号	1660-3397
DOI	10.3390/md7010024
文献子类	Article
英文摘要	The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.
资助项目	The National High Technology Development Project (863)[2007AA09Z415] ; The National Natural Science Foundation of China, Guangdong Province Union Key Project[U0633008] ; Program of New Century Excellent Talents, China Ministry of Education[NCET-05-0756] ; Program for Social Profit, China Ministry of Science and Technology[2004DIB3J072]
WOS关键词	TYROSINE-PHOSPHATASE 1B ; SP-NOV. ; SELECTIVE ISOLATION ; MARIANA TRENCH ; SECONDARY METABOLITES ; GENOME SEQUENCE ; GEN. NOV. ; STREPTOMYCES ; DIVERSITY ; SEDIMENTS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI
WOS记录号	WOS:000264557700004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279305]
专题	国家新药筛选中心药物安全性评价中心
通讯作者	Hong, Kui
作者单位	1.Newcastle Univ, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England; 2.Chinese Acad Sci, Inst Microbiol, Beijing 100081, Peoples R China 3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China; 4.Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China; 5.Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Hong, Kui,Gao, An-Hui,Xie, Qing-Yi,et al. Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China[J]. MARINE DRUGS,2009,7(1):24-44.
APA	Hong, Kui.,Gao, An-Hui.,Xie, Qing-Yi.,Gao, Hao.,Zhuang, Ling.,...&Ruan, Ji-Sheng.(2009).Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China.MARINE DRUGS,7(1),24-44.
MLA	Hong, Kui,et al."Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China".MARINE DRUGS 7.1(2009):24-44.