CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS

doi:10.1016/j.neuroscience.2016.09.001

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	CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS
	Zhang, Lijun 1; Hua, Qiuhong 1; Tang, Kaiyi 1; Shi, Changjie 1; Xie, Xin1,2 ; Zhang, Ru 1
刊名	NEUROSCIENCE
	2016-11-19
卷号	337 页码:88-97
关键词	human embryonic stem cell neural stem/precursor cell neural differentiation chemokine receptor CXCR4 SDF-1
ISSN号	0306-4522
DOI	10.1016/j.neuroscience.2016.09.001
文献子类	Article
英文摘要	G protein-coupled receptors (OGPCRs) are involved in many fundamental cellular responses such as growth, death, movement, transcription and excitation. Their roles in human stem cell neural specialization are not well understood. In this study, we aimed to identify GPCRs that may play a role in the differentiation of human embryonic stem cells (OhESCs) to neural stem cells (ONSCs). Using a feeder-free hESC neural differentiation protocol, we found that the expression of several chemokine receptors changed dramatically during the hESC/NSC transition. Especially, the expression of CXCR4 increased approximately 50 folds in NSCs compared to the original hESCs. CXCR4 agonist SDF-1 promoted, whereas the antagonist AMD3100 delayed the neural induction process. In consistence with antagonizing CXCR4, knockdown of CXCR4 in hESCs also blocked the neural induction and cells with reduced CXCR4 were rarely positive for Nestin and Sox1-staining. Taken together, our results suggest that CXCR4 is involved in the neural induction process of hESC and it might be considered as a target to facilitate NSC production from hESCs in regenerative medicine. (C) 2016 The Authors. Published by Elsevier Ltd on behalf of IBRO. This is an open access article under the CCBY-NC-ND license.
资助项目	Ministry of Science and Technology of China[2014CB965002] ; Ministry of Science and Technology of China[2015CB964503] ; Science and Technology Commission of Shanghai Municipality, China[15JC1400202] ; Chinese Academy of Sciences, China[XDA01040301] ; National Natural Science Foundation of China[81425024] ; National Natural Science Foundation of China[31371511]
WOS关键词	PROTEIN-COUPLED RECEPTORS ; CHEMOKINE RECEPTOR ; PROGENITOR CELLS ; ADULT NEUROGENESIS ; SIGNALING PATHWAY ; DENTATE GYRUS ; IN-VITRO ; MIGRATION ; PROLIFERATION ; CXCL12
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000386337600008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275803]
专题	国家新药筛选中心
通讯作者	Xie, Xin; Zhang, Ru
作者单位	1.Tongji Univ, Sch Life Sci & Technol, Collaborat Innovat Ctr Brain Sci, Shanghai Key Lab Signaling & Dis Res,Lab Receptor, Shanghai 200092, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lijun,Hua, Qiuhong,Tang, Kaiyi,et al. CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS[J]. NEUROSCIENCE,2016,337:88-97.
APA	Zhang, Lijun,Hua, Qiuhong,Tang, Kaiyi,Shi, Changjie,Xie, Xin,&Zhang, Ru.(2016).CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS.NEUROSCIENCE,337,88-97.
MLA	Zhang, Lijun,et al."CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS".NEUROSCIENCE 337(2016):88-97.