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	Detection of Membrane Protein Interactions by Cell-based Tango Assays
	Yan, Yan3; Xu, Ting-Hai3; Harikumar, Kaleeckal G2; Miller, Laurence J2; Melcher, Karsten1; Xu, H Eric3
刊名	Bio-protocol
	2017-11-20
卷号	7期号:22
ISSN号	2331-8325
DOI	10.21769/BioProtoc.2903
文献子类	Article
英文摘要	The Tango assay is a protein-protein interaction assay, in which a transcription factor (rTA) is fused to a membrane-bound protein via a linker that contains a cleavage site for TEV protease, whereas a soluble interaction partner is fused to TEV protease (Barnea , 2008). Association between the two interaction partners leads to an efficient cleavage of the transcription factor, allowing it to translocate to the nucleus and activate a luciferase reporter gene as measurement of the interactions. In this modified assay, we fused one copy of the membrane-spanning amyloid precursor protein (APP) C99 region to TEV site-rTA (C99-TEV site-rTA) and a second copy to TEV protease (C99-TEV) to analyze intramembrane C99-C99 interaction in live cells.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266515]
专题	药物靶标结构与功能中心
作者单位	1.Center for Cancer and Cell Biology, Innovation and Integration Program, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA 2.Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA; 3.Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
推荐引用方式 GB/T 7714	Yan, Yan,Xu, Ting-Hai,Harikumar, Kaleeckal G,et al. Detection of Membrane Protein Interactions by Cell-based Tango Assays[J]. Bio-protocol,2017,7(22).
APA	Yan, Yan,Xu, Ting-Hai,Harikumar, Kaleeckal G,Miller, Laurence J,Melcher, Karsten,&Xu, H Eric.(2017).Detection of Membrane Protein Interactions by Cell-based Tango Assays.Bio-protocol,7(22).
MLA	Yan, Yan,et al."Detection of Membrane Protein Interactions by Cell-based Tango Assays".Bio-protocol 7.22(2017).

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