Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells

doi:10.21769/BioProtoc.2904

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	Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells
	Harikumar, Kaleeckal G 1; Yan, Yan 3; Xu, Ting-Hai 3; Melcher, Karsten 2; Xu, H Eric 3; Miller, Laurence J 1
刊名	Bio-protocol
	2017-11-20
卷号	7 期号:22
ISSN号	2331-8325
DOI	10.21769/BioProtoc.2904
文献子类	Article
英文摘要	The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap with the donor emission, is approximated with the donor. In the example provided, proteins tagged with bioluminescent luciferase (Rlu) as donor and yellow fluorescent protein (YFP) as acceptor were co-expressed in cells. This pair of donor and acceptor have an approximate Förster distance of 4.4 nm, providing the optimal working distance (Dacres ., 2010). This technique can be used to explore the time-course of specific molecular interactions that occur in living cells.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266514]
专题	药物靶标结构与功能中心
作者单位	1.Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA; 2.University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China 3.Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
推荐引用方式 GB/T 7714	Harikumar, Kaleeckal G,Yan, Yan,Xu, Ting-Hai,et al. Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells[J]. Bio-protocol,2017,7(22).
APA	Harikumar, Kaleeckal G,Yan, Yan,Xu, Ting-Hai,Melcher, Karsten,Xu, H Eric,&Miller, Laurence J.(2017).Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells.Bio-protocol,7(22).
MLA	Harikumar, Kaleeckal G,et al."Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells".Bio-protocol 7.22(2017).