Restoration of sternness-high tumor cell-mediated suppression of murine dendritic cell activity and inhibition of tumor growth by low molecular weight oyster polysaccharide

doi:10.1016/j.intimp.2018.10.003

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	Restoration of sternness-high tumor cell-mediated suppression of murine dendritic cell activity and inhibition of tumor growth by low molecular weight oyster polysaccharide
	Zhong, Ming 2; Zhong, Cheng 3; Hu, Pei 1; Cui, Wen 2; Wang, Guanghui 2; Gao, Huijei 2; Liu, Chao 2; Liu, Zhiqiang 2; Li, Zhihua 2; Li, Chunxia 2
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2018-12
卷号	65 页码:221-232
关键词	Colorectal cancer stem cells Dendritic cells TGF-beta NF-kappa B Oyster polysaccharide
ISSN号	1567-5769
DOI	10.1016/j.intimp.2018.10.003
文献子类	Article
英文摘要	Dendritic cells (DCs) play key regulatory roles in tumor immunity: increased activity of DCs infiltrating tumor tissues leads to enhancement of tumor immunity. Functions of DCs are also modulated by tumor cell-derived factors. Here, we investigated the effects of low molecular weight oyster polysaccharide (LMW-OPS) on differentiation and function of bone marrow-derived DCs (BMDCs) exposed to a conditioned medium (CM) obtained from spheres of sternness-high colorectal cancer cell lines CMT93 and CT26. The CM containing a detectable level of TGF-beta 1 was found to down-regulate the surface expression of major histocompatibility complex class II of BMDCs and to inhibit the potency of BMDCs to stimulate T cells. Those suppressions were partly restored and completely restored by addition of anti-TGF-beta 1 and LMW-OPS, respectively. Production of IFN-gamma during allogeneic T cell responses was inhibited by the CM, whereas production of TGF-beta 1 was augmented by the CM. The IFN-gamma profile was also reversed by addition of LMW-OPS. Nuclear translocation of beta-catenin, but not that of NF-kappa B p65, was induced by TGF-beta 1. NF-kappa B p65 nuclear translocation, but not beta-catenin nuclear trans location, was induced by LMW-OPS. Intraperitoneal injection of LMW-OPS significantly suppressed tumor growth in syngeneic tumor models using CMT93 and CT26 sphere cells, whereas it had no inhibitory effect on the proliferation of either cell line. The results demonstrated that LMW-OPS relieved sternness-high tumor cell mediated suppression of BMDC function and indicated the in vivo anti-tumor activity of LMW-OPS in which re stimulation of the activity of DCs infiltrating tumor tissues is presumed to be involved.
资助项目	Shandong Provincial Natural Science Foundation, China[ZR2014HM038]
WOS关键词	CANCER STEM-CELLS ; NF-KAPPA-B ; GLIOMA-INITIATING CELLS ; TGF-BETA ; ANTITUMOR IMMUNITY ; T-CELLS ; MATURATION ; HOMEOSTASIS ; ACTIVATION ; TOLERANCE
WOS研究方向	Immunology ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000452940500025
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279474]
专题	上海中药现代化研究中心
通讯作者	Zhong, Ming; Hu, Pei
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Haike Rood 501, Shanghai, Peoples R China; 2.Jining Med Coll, Inst Tumor Pharmacol, Xueyuan Rd 669, Rizhao 276826, Peoples R China; 3.Univ Tokyo, Div Stem Cell Dynam, Ctr Stem Cell Biol & Regenerat Med, Inst Med Sci, Tokyo, Japan; 4.Okayama Univ, Div Pharmaceut Sci, Grad Sch Med Dent & Pharmaceut Sci, Okayama, Japan
推荐引用方式 GB/T 7714	Zhong, Ming,Zhong, Cheng,Hu, Pei,et al. Restoration of sternness-high tumor cell-mediated suppression of murine dendritic cell activity and inhibition of tumor growth by low molecular weight oyster polysaccharide[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2018,65:221-232.
APA	Zhong, Ming.,Zhong, Cheng.,Hu, Pei.,Cui, Wen.,Wang, Guanghui.,...&Gohda, Eiichi.(2018).Restoration of sternness-high tumor cell-mediated suppression of murine dendritic cell activity and inhibition of tumor growth by low molecular weight oyster polysaccharide.INTERNATIONAL IMMUNOPHARMACOLOGY,65,221-232.
MLA	Zhong, Ming,et al."Restoration of sternness-high tumor cell-mediated suppression of murine dendritic cell activity and inhibition of tumor growth by low molecular weight oyster polysaccharide".INTERNATIONAL IMMUNOPHARMACOLOGY 65(2018):221-232.