A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui

doi:10.1016/j.jpba.2013.08.049

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	A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui
	Hou, Jin-Jun1,2 ; Wu, Wan-Ying1 ; Liang, Jian 3; Yang, Zhou 1; Long, Hua-Li 1; Cai, Lu-Ying 1; Fang, Lin 2; Wang, Dan-Dan 1; Yao, Shuai 1; Liu, Xuan 1
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2014-01-25
卷号	88 页码:321-330
关键词	Single standard to determine multi-components (SSDMC) Euphorbia kansui Exogenous reference standard Ingenane diterpenoids Jatrophane diterpenoids
ISSN号	0731-7085
DOI	10.1016/j.jpba.2013.08.049
文献子类	Article
英文摘要	Kansui radix is a famous poisonous traditional Chinese medicine. However, due to its different types of constituents with broad polarity, a variety of UV absorptions and lack of the reference standards, it was difficult to simultaneously determine the main component in kanui radix. A single, multi-faceted, enhanced strategy, exogenous reference standard - single standard to determine multi-components method (ERS-SSDMC), was proposed. Thirteen major components of kansui radix, including three jatrophane diterpenoids, eight ingenane diterpenoids and two triterpenes, among which there were three pairs of isomers, were simultaneously assayed. A C8 column, packed with 2.7 2.7 mu m core-shell particles, was optimized to separate these constituents in 25 min on HPLC instrument detected at a program wavelength. Ethyl benzoate employed as single exogenous reference standard. The method was fully validated with respect to linearity (r(2) > 0.9995), LOQs (0.1-0.41 mu g/mL), precision, accuracy (92-114%, RSD < 4.4%) and stability. The robustness of the method was performed by Plackett-Burmantest tests which eight primary chromatographic parameters were investigated. It was found that the two factors, wavelength and flow rate, should be strictly controlled. A total of 75 batches of kansui radix and its three different processing products were successfully analyzed and discriminated by applying the proposed method. This work demonstrates an effective strategy for the SSDMC method making the simultaneous assay of complex multi-component TCM system achievable. (C) 2013 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81073026]
WOS关键词	STANDARD
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000329532800043
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277221]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-Ying
作者单位	1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China; 3.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Hou, Jin-Jun,Wu, Wan-Ying,Liang, Jian,et al. A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,88:321-330.
APA	Hou, Jin-Jun.,Wu, Wan-Ying.,Liang, Jian.,Yang, Zhou.,Long, Hua-Li.,...&Guo, De-An.(2014).A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,88,321-330.
MLA	Hou, Jin-Jun,et al."A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 88(2014):321-330.