Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS

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	Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS
	LIANG Guang-yan 2; WU Yun-shan 3; CHEN Wei-ying 3; LIU Bo 2,3; GUO De-an1,2,3
刊名	Chinese Journal of Experimental Traditional Medical Formulae
	2018
卷号	24 期号:1 页码:67-73
关键词	brazilin Sappan Lignum metabolites sulfation glucuronide conjugation urine bile
ISSN号	1005-9903
其他题名	巴西苏木素在大鼠体内的代谢产物与代谢途径分析
文献子类	Article
英文摘要	Objective:The metabolites of brazilin were detected and identified in vivo. Method:The metabolic profiles of brazilin in plasma,urine,bile and feces samples after oral administration (30 mg·kg~(-1)) were analyzed by UPLC-Q-TOF-MS/MS.The mobile phase was consisted of water(containing of 0.1% formic acid)-acetonitrile and eluted in gradient mode.The mass was detected in negative ion mode and information dependent acquisition(IDA) model was used.The data were analyzed by PeakView 1.2 software.Total ion chromatogram of sample was extracted and compared with the blank biological sample,possible metabolites were screened and identified by comparison and analysis of retention time,precise molecular mass,elemental composition and secondary mass spectrometry. Result:Twenty-three metabolites of brazilin were detected by comparing with the blank sample.The pathways to these metabolites were methylation,deoxygenation,hydrogenation,carboxylation,hydration,sulfation and glucuronide conjugation,in which sulfation and glucuronide conjugation were the main transitions. Conclusion:This study provides the metabolism of brazilin in rats for the first time,which can be useful for its pharmacology and pharmacodynamics.
资助项目	国家自然科学基金项目[00000000] ; 广东省科技计划项目[00000000] ; 广东省中医院中医药科学技术研究专项[00000000] ; 国家中医药管理局临床基地科研专项[00000000] ; 广东省高水平大学建设项目[00000000]
WOS研究方向	Pharmacology & Pharmacy ; General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:6158456
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269181]
专题	上海中药现代化研究中心
通讯作者	GUO De-an
作者单位	1.Shanghai Research Center of Modernization of Chinese Materia Medica, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China 2.School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China; 3.The 2^nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China;
推荐引用方式 GB/T 7714	LIANG Guang-yan,WU Yun-shan,CHEN Wei-ying,et al. Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS[J]. Chinese Journal of Experimental Traditional Medical Formulae,2018,24(1):67-73.
APA	LIANG Guang-yan,WU Yun-shan,CHEN Wei-ying,LIU Bo,&GUO De-an.(2018).Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS.Chinese Journal of Experimental Traditional Medical Formulae,24(1),67-73.
MLA	LIANG Guang-yan,et al."Metabolites and Metabolic Pathway Analysis of Brazilin in Rats by UPLC-Q-TOF-MS/MS".Chinese Journal of Experimental Traditional Medical Formulae 24.1(2018):67-73.