Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry

doi:10.1016/j.jpba.2018.12.013

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry
	Yisimayili, Zainaipuguli 1,2,3; Guo, Xiaozhen 2,3; Liu, Huan 2,3; Xu, Zhou 4; Abdulla, Rahima 1; Akber Aisa, Haji 1,3; Huang, Chenggang3,4
刊名	Journal of pharmaceutical and biomedical analysis
	2018-12-10
卷号	165 页码:251-260
ISSN号	1873-264X
DOI	10.1016/j.jpba.2018.12.013
文献子类	Article
英文摘要	Corilagin is an Ellagitannin with favorable pharmacological activities. But there was no report regarding the metabolism of corilagin in vitro or in vivo. In this study, the metabolic profile of corilagin in rats as well as in rat intestinal bacteria and liver microsomes incubation system in vitro were investigated comprehensively for the first time. Consequently, with the aid of sensitive HPLC-Q-TOF-MS/MS, corilagin and its twenty-four metabolites (fourteen phase II conjugate metabolites of corilagin, three hydrolyzed metabolites EA, GA, M3 and their seven derived metabolites) were absolutely or tentatively identified in rat biological samples (urine, feces, plasma and tissues) after oral administration of corilagin. In vitro, the three hydrolyzed metabolites were identified in rat intestinal microflora and liver microsomes. These results demonstrated that corilagin itself not only could underwent extensive phase II metabolism in rats, but also could underwent hydrolysis reaction in rats as well as in rat intestinal bacteria and liver microsomes in vitro. This study is first report to identify phase II conjugate metabolites (except mono-methylate conjugated metabolites) of pure Ellagitannin and distribution of these metabolites in vivo. In addition, clear, detailed metabolic pathways of corilagin were shown to involve hydrolysis, methylation, glycosylation, reduction, glucuronidation and sulfation.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266347]
专题	上海中药现代化研究中心
通讯作者	Akber Aisa, Haji; Huang, Chenggang
作者单位	1.Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumchi 830011, China; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 3.University of Chinese Academy of Sciences, Beijing 100049, China; 4.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
推荐引用方式 GB/T 7714	Yisimayili, Zainaipuguli,Guo, Xiaozhen,Liu, Huan,et al. Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry[J]. Journal of pharmaceutical and biomedical analysis,2018,165:251-260.
APA	Yisimayili, Zainaipuguli.,Guo, Xiaozhen.,Liu, Huan.,Xu, Zhou.,Abdulla, Rahima.,...&Huang, Chenggang.(2018).Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry.Journal of pharmaceutical and biomedical analysis,165,251-260.
MLA	Yisimayili, Zainaipuguli,et al."Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry".Journal of pharmaceutical and biomedical analysis 165(2018):251-260.