A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging

doi:10.7150/thno.11139

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物制剂研究中心

	A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging
	Wang, Yaping 1,2; Lin, Tingting 1,2; Zhang, Wenyuan 2; Jiang, Yifan 2; Jin, Hongyue 2; He, Huining 1; Yang, Victor C.1,3; Chen, Yi2 ; Huang, Yongzhuo2
刊名	THERANOSTICS
	2015
卷号	5 期号:8 页码:787-795
关键词	matrix metalloprotease protease-activatable probe tumor imaging brain tumor fluorescence resonance energy transfer
ISSN号	1838-7640
DOI	10.7150/thno.11139
文献子类	Article
英文摘要	Tumor-associated proteases (TAPs) have been intensively studied because of their critical roles in cancer development. As a case in point, expression of matrix metalloproteases (MMP) is significantly up-regulated in tumorigenesis, invasion, and metastasis among a majority of cancers. Here we present a prodrug-type, MMP-2-responsive nanoprobe system with high efficiency and low toxicity for detecting MMP-2-overexpressed tumors. The nanoprobe system is featured by its self-assembled fabrication and FRET effect. This prodrug-type nanoprobe is selectively activated by MMP-2, and thus useful for detection of the MMP-2-overexpressed cells and tumors. The nanoprobe system works successfully in various animal tumor models, including human fibrosarcoma and subcutaneous glioma xenograft. Furthermore, in order to overcome the blood brain barrier (BBB) and achieve brain tumor targeting, a transferrin-receptor targeting peptide (T7 peptide) is strategically incorporated into the nanoprobe. The T7-functionalized nanoprobe is capable of detecting the orthotopic brain tumor, with clear, real-time in vivo imaging. This method is promising for in vivo detection of brain tumor, and real-time monitor of a TAP (i.e., MMP-2).
资助项目	National Basic Research Program of China (973 Program)[2013CB932503] ; National Basic Research Program of China (973 Program)[2014CB931900] ; NSFC of China[91029743] ; NSFC of China[81172996] ; NSFC of China[81373357] ; NSFC of China[81361140344] ; NSFC of China[81422048]
WOS关键词	MATRIX METALLOPROTEINASE-2 ; NANOPARTICLES ; CANCER ; EXPRESSION ; GLIOMA
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000354959900001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276732]
专题	药物制剂研究中心
通讯作者	Huang, Yongzhuo
作者单位	1.Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Univ Michigan, Coll Pharm, Ann Arbor, MI 48108 USA
推荐引用方式 GB/T 7714	Wang, Yaping,Lin, Tingting,Zhang, Wenyuan,et al. A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging[J]. THERANOSTICS,2015,5(8):787-795.
APA	Wang, Yaping.,Lin, Tingting.,Zhang, Wenyuan.,Jiang, Yifan.,Jin, Hongyue.,...&Huang, Yongzhuo.(2015).A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging.THERANOSTICS,5(8),787-795.
MLA	Wang, Yaping,et al."A Prodrug-type, MMP-2-targeting Nanoprobe for Tumor Detection and Imaging".THERANOSTICS 5.8(2015):787-795.