Determination of the interaction kinetics between meloxicam and beta-cyclodextrin using the quantitative high-performance affinity chromatography coupled with mass spectrometry

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	Determination of the interaction kinetics between meloxicam and beta-cyclodextrin using the quantitative high-performance affinity chromatography coupled with mass spectrometry
	Wang Caifen 1; Li Zhuo 1; Wang Xiaobo 1; Li Haiyan2 ; Zhang Jiwen1 ; Sun Lixin 1
刊名	Acta Pharmaceutica Sinica
	2015
卷号	50 期号:9 页码:1167-1173
关键词	quantitative high performance affinity chromatography-mass spectrometry meloxicam beta- cyclodextrin dissociation rate constant
ISSN号	0513-4870
其他题名	定量亲和色谱-质谱研究美洛昔康与beta-环糊精的相互作用
文献子类	Article
英文摘要	The association rate constant and dissociation rate constant are important parameters of the drug-cyclodextrin supermolecule systems, which determine the dissociation of drugs from the complex and the further in vivo absorption of drugs. However, the current studies of drug-cyclodextrin interactions mostly focus on the thermodynamic parameter of equilibrium constants(K). In this paper, a method based on quantitative high performance affinity chromatography coupled with mass spectrometry was developed to determine the apparent dissociation rate constant(k_(off,app)) of drug-cyclodextrin supermolecule systems. This method was employed to measure the k_(off,app) of meloxicam and acetaminophen. Firstly, chromatographic peaks of drugs and non-retained solute(uracil) on beta-cyclodextrin column at different flow rates were acquired, and the retention time and variance values were obtained via the fitting the peaks. Then, the plate heights of drugs(H_R) and uracil(H_(M,C)) were calculated. The plate height of theoretical non-retained solute(H_(M,T)) was calculated based on the differences of diffusion coefficient and the stagnant mobile phase mass transfer between drugs and uracil. Finally, the k_(off,app) was calculated from the slope of the regression equation between(H_R-H_(M,T)) and uk/(1+k)~2,(0.13 0.00) s~(-1) and(4.83 0.10) s~(-1) for meloxicam and acetaminophen(control drug), respectively. In addition, the apparent association rate constant(k_(on,app)) was also calculated through the product of K(12.53 L·mol~(-1)) and k_(off,app). In summary, it has been proved that the method established in our study was simple, efficiently fast and reproducible for investigation on the kinetics of drug-cyclodextrin interactions.
资助项目	国家自然科学基金资助项目[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:5512075
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269272]
专题	药物制剂研究中心
通讯作者	Zhang Jiwen
作者单位	1.Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	Wang Caifen,Li Zhuo,Wang Xiaobo,et al. Determination of the interaction kinetics between meloxicam and beta-cyclodextrin using the quantitative high-performance affinity chromatography coupled with mass spectrometry[J]. Acta Pharmaceutica Sinica,2015,50(9):1167-1173.
APA	Wang Caifen,Li Zhuo,Wang Xiaobo,Li Haiyan,Zhang Jiwen,&Sun Lixin.(2015).Determination of the interaction kinetics between meloxicam and beta-cyclodextrin using the quantitative high-performance affinity chromatography coupled with mass spectrometry.Acta Pharmaceutica Sinica,50(9),1167-1173.
MLA	Wang Caifen,et al."Determination of the interaction kinetics between meloxicam and beta-cyclodextrin using the quantitative high-performance affinity chromatography coupled with mass spectrometry".Acta Pharmaceutica Sinica 50.9(2015):1167-1173.