Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile

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	Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile
	Hong Liling 1; Wang Ting 1; Fan Bin 1; Zhou Qingyun 1; Huang Yongzhuo3 ; Wang Feng 2
刊名	Carcinogenesis, Teratogenesis and Mutagenesis
	2015
卷号	27 期号:6 页码:450
关键词	dibromo acetonitrile genotoxicity apoptosis cytokinesis-block micronucleus cytome assays flow cytometry
ISSN号	1004-616X
其他题名	二溴乙腈对L5178Y细胞的遗传毒性及促凋亡作用
文献子类	Article
英文摘要	OBJECTIVE: To study the genotoxicity and apoptosis of the mice L5178Y lymphoma cells which was induced by disinfection by-product dibromo acetonitrile in drinking water. METHODS:Cytokinesis-block micronucleus cytome assay (CBMN-cyt) and flow cytometry were used to evaluate genotoxicity and apoptosis in mice L5178Y lymphoma cells treated with different concentrations of dibromo acetonitrile (0.1,1,5 and 10 mol/L). RESULTS:Compared with the negative control group,frequency of micronucleus (MN) of L5178Y lymphoma cells in 1 and 5 mumol/L groups increased significantly (P<0.05). The nucleoplasmic bridges (NPBs) in the 1 and 10 mumol/L treatment groups and the frequency of nuclear buds (NBUDs) in the 10 mumol/L treatment group all increased significantly (P<0.05). However the nuclear divided index (NDI) in the 5 and 10 mumol/L treatment groups all decreased significantly (P<0.05). Statistically significant increase of L5178Y lymphoma cell apoptosis were observed in all dibromo acetonitrile treatment groups in comparison to the negative control group (P<0.05). CONCLUSION:Disinfection by-product dibromo acetonitrile in drinking water could obviously induce genotoxicity and apoptosis in L5178Y lymphoma cells.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:5583398
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269264]
专题	药物制剂研究中心
作者单位	1.Toxicology Laboratory, Testing Center of Shanghai Research Institute of Chemical Industry, Shanghai 200062, China.; 2.Amway Research & Development Center, Shanghai 201203, China. 3.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.;
推荐引用方式 GB/T 7714	Hong Liling,Wang Ting,Fan Bin,et al. Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile[J]. Carcinogenesis, Teratogenesis and Mutagenesis,2015,27(6):450.
APA	Hong Liling,Wang Ting,Fan Bin,Zhou Qingyun,Huang Yongzhuo,&Wang Feng.(2015).Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile.Carcinogenesis, Teratogenesis and Mutagenesis,27(6),450.
MLA	Hong Liling,et al."Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile".Carcinogenesis, Teratogenesis and Mutagenesis 27.6(2015):450.